CPT1A Overexpression Lysate (Native)

Images

 
Western Blot: CPT1A Overexpression Lysate (Adult Normal) [NBL1-09449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPT1A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CPT1A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200485 and based on accession number NM_001031847. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 2, mRNA.
Gene
CPT1A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
86.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CPT1A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CPT1A Overexpression Lysate (Native)

  • carnitine O-palmitoyltransferase 1, liver isoform
  • Carnitine O-palmitoyltransferase I, liver isoform
  • carnitine palmitoyltransferase 1A (liver)
  • Carnitine palmitoyltransferase 1A
  • carnitine palmitoyltransferase I, liver
  • CPT I
  • CPT1
  • CPT1-LEC 2.3.1.21
  • CPTI-L
  • EC 2.3.1
  • L-CPT1

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) contains an alternative 3' terminal exon compared to transcript variant 1. This results in a shorter isoform (2) with a different C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CPT1A Lysate (NBL1-09449) (0)

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Video Protocols

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Bioinformatics

Gene Symbol CPT1A