CPT1A Antibody (HL1164) - Azide and BSA Free

Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog intestine. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat tissues. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Ca, Fe
• Applications: WB, IHC
• Clone: HL1164
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CPT1A Antibody (HL1164) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CPT1A Antibody (HL1164) - Azide and BSA Free (NBP3-25423) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CPT1A. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CPT1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%), Sheep (85%), Bovine (86%), Porcine (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CPT1A Antibody (HL1164) - Azide and BSA Free

• carnitine O-palmitoyltransferase 1, liver isoform
• Carnitine O-palmitoyltransferase I, liver isoform
• carnitine palmitoyltransferase 1A (liver)
• Carnitine palmitoyltransferase 1A
• carnitine palmitoyltransferase I, liver
• CPT I
• CPT1
• CPT1A
• CPT1-L
• CPT1-LEC 2.3.1.21
• CPTI-L
• EC 2.3.1
• EC 2.3.1.21
• L-CPT1

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPT1A