COUP-TF I/NR2F1 Antibody - BSA Free

Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

COUP-TF I/NR2F1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit COUP-TF I/NR2F1 Antibody - BSA Free (NBP2-15974) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human COUP-TF I/NR2F1. The exact sequence is proprietary.
• Predicted Species: Rat (99%), Xenopus (98%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR2F1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: Immunoprecipitation Assay-dependent dilution
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for COUP-TF I/NR2F1 Antibody - BSA Free

• COUP transcription factor 1
• COUPTF I
• COUP-TF I
• COUP-TF1
• COUP-TFI
• EAR-3COUP transcription factor I
• EAR3COUP-TF I
• ERBAL3
• ERBAL3V-erbA-related protein 3
• NR2F1
• Nuclear receptor subfamily 2 group F member 1
• nuclear receptor subfamily 2, group F, member 1
• SVP44
• TCFCOUP1
• TFCOUP1NR2F2
• transcription factor COUP 1 (chicken ovalbumin upstream promoter 1, v-erb-ahomolog-like 3)

Background

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NR2F1
• Uniprot:
  • P10589(Human)