NrCAM Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NrCAM Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NrCAM isoform 3
  Leu30-Asn600 (Ala526Pro)
  Accession # Q14CA1
• Specificity: Detects human NrCAM Isoform 3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRCAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NrCAM Antibody [Unconjugated]

• Bravo
• hBravo
• KIAA0343Neuronal surface protein Bravo
• MGC138845
• MGC138846
• neuronal cell adhesion molecule
• NgCAM-related cell adhesion molecule
• ng-CAM-related
• NrCAM
• nr-CAM

Background

NgCAM related cell adhesion molecule (NrCAM), also known as Bravo, belongs to the L1 family of cell adhesion molecules, which also include L1, neurofascin and close homolog of L1 (CHL-1) (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like domains in their extracellular domain. They also shared a conserved cytoplasmic region containing an ankyrin-binding site. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (1, 2). NrCam mediates homophilic adhesion as well as heterophilic adhesion with a number of neuronal adhesion molecules including contactin, TAG-1/contactin-2, neurofascin and receptor tyrosine phosphatase beta (RPTP beta ) (3‑5). NrCAM has been implicated in the axogenesis of multiple neuronal populations including sensory axons and commissural axons in the spinal cord of chick embryos. It also plays a role in cerebellar granule cell development, and is required in node of Ranvier formation (5, 6).

1. Hortsch, M. (2000) Mol. Cell. Neurosci. 15:1.
2. Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.
3. Mauro, V.P. et al. (1992) 119:191.
4. Fustig, M. Sakurai, T. and M. Grumet (1999) Dev. Bio. 209:340.
5. Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.
6. Custer, A.W. et al. (2003) J. Neuroscience 23:10032.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NrCAM Antibody (AF2034)(3)

Publications using AF2034

• Robin Wachowiak, Steffi Mayer, Anne Suttkus, Illya Martynov, Martin Lacher, Nathaniel Melling et al. CHL1 and NrCAM are primarily expressed in low grade pediatric neuroblastoma Open Med (Wars) 2019-12-31 [PMID: 31989042] (Immunohistochemistry, Human)
• Robin Wachowiak, Steffi Mayer, Anne Suttkus, Illya Martynov, Martin Lacher, Nathaniel Melling et al. CHL1 and NrCAM are primarily expressed in low grade pediatric neuroblastoma Open Med (Wars) 12/31/2019 [PMID: 31989042]
• Tsourlakis , M C, Walter , E, Quaas , A, Graefen , M, Huland , H, Simon , R, Sauter , G, Steurer , S, Schlomm , T, Minner , S High Nr-CAM expression is associated with favorable phenotype and late PSA recurrence in prostate cancer treated by prostatectomy. Prostate Cancer Prostatic Dis, 2013-01-22;16(2):159-64. 2013-01-22 [PMID: 23338556] (IHC, Human)

Bioinformatics

• Gene Symbol: NRCAM
• Uniprot:
  • Q14CA1()