COMT Antibody

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Western Blot: COMT Antibody [NB100-41387] - Staining of human testis lysate (35ug protein in RIPA buffer) with Goat Anti-COMT (internal) (0.03ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - (4ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA: COMT Antibody [NB100-41387] - 0.5ug/ml) as the reporter with the capture rabbit antibody (2.5ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, EIA, ELISA, IHC, IHC-P, PEP-ELISA, S-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

COMT Antibody Summary

Immunogen
Peptide with sequence C-QDIIPQLKKKYDVD corresponding to internal region according to NP_000745.1, NP_009294.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COMT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • ELISA 0.5-2ug/ml
  • Enzyme Immunoassay 0.5 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Sandwich ELISA 0.5 - 2 ug/ml
Application Notes
Western blot: Approx 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1).
IHC: In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Successfully used in Paraffin embedded Human Testis and Small Intestine.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody.
Control
COMT Knockout 293T Cell Lysate
Publications
Read Publication using NB100-41387.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COMT Antibody

  • catechol O-methyltransferase
  • catechol-O-methyltransferase
  • COMT
  • EC 2.1.1.6

Background

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

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Bioinformatics

Gene Symbol COMT
Entrez
Uniprot