COMT Antibody

Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.

Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Cerebellum. Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

COMT Antibody Summary

• Description: Novus Biologicals Goat COMT Antibody (NB100-41387) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-COMT Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QDIIPQLKKKYDVD corresponding to internal region according to NP_000745.1, NP_009294.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000745.1 and NP_009294.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: COMT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/mL
• Application Notes: Western blot: Approx 28 kDa band in Human Testis lysates (calculated MW of 30.0 kDa according to NP_000745.1 and 24.5 kDa according to NP_009294.1). IHC: In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration: 2 - 4 ug/mL. Paraffin embedded Human Testis and Small Intestine. Recommended concentration: 3.75 ug/mL.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COMT Antibody

• catechol O-methyltransferase
• catechol-O-methyltransferase
• COMT
• EC 2.1.1.6

Background

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COMT Antibody (NB100-41387)(1)

Publication using NB100-41387

• Tunbridge EM, Harrison PJ, Weinberger DR. Catechol-o-Methyltransferase, Cognition, and Psychosis: Val(158)Met and Beyond. Biol Psychiatry 2006-02-11 [PMID: 16476412]

Bioinformatics

• Gene Symbol: COMT
• Entrez:
  • 1312(Human)
• Uniprot:
  • NP_000745.1(Human)