COMT Antibody (JG39-48)

Western Blot: COMT Antibody (JG39-48) [NBP2-75444] - Analysis of COMT on MCF-7 cell (1) and rat testis tissue (2) lysate using anti-COMT antibody at 1/2,000 dilution.

Immunohistochemistry-Paraffin: COMT Antibody (JG39-48) [NBP2-75444] - Analysis of paraffin-embedded mouse testis tissue using anti-COMT antibody. Counter stained with hematoxylin.

Product Discontinued

COMT Antibody (JG39-48) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within COMT aa 11-107 / 271. (SwissProt: P21964 Human; SwissProt: O88587 Mouse; SwissProt: P22734 Rat)
• Localization: Cell membrane. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: COMT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for COMT Antibody (JG39-48)

• catechol O-methyltransferase
• catechol-O-methyltransferase
• COMT
• EC 2.1.1.6

Background

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COMT