Complement Factor B Antibody (9B8) - BSA Free

Product Discontinued

Complement Factor B Antibody (9B8) - BSA Free Summary

• Immunogen: Complement factor B purified from human plasma and adsorbed to aluminum hydroxide gel
• Epitope: Differs from that of Clone 06. Also differs from Clone 04 and NBP1-05060, but with slight overlap as determined by some mutual inhibition of antibody binding.
• Specificity: Clone 9B8 is specific for the Bb fragment of human factor B. Antibody binding abolishes the formation of C3bBb complexes.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 9B8 reacts strongly with human factor B. A strong reaction is seen when used as a biotinylated detection antibody in sandwich ELISA with a polyclonal capture antibody against factor B . In Western blotting after SDS-PAGE, Clone 9B8 reacts with the Bb fragment of factor B in its non-reduced form only. Clone 9B8 can be used for the affinity purification of human factor B, when coupled to a suitable matrix.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement Factor B Antibody (9B8) - BSA Free

• BF AHUS4
• BF
• B-factor, properdin
• BFD
• C3 proaccelerator
• C3 proactivator
• C3/C5 convertase
• CFAB
• CFB
• Complement Factor B
• EC 3.4.21
• EC 3.4.21.47
• FB
• FBI12
• GBG
• GBGCFAB
• Glycine-rich beta glycoprotein
• glycine-rich beta-glycoprotein
• H2-Bf
• PBF2
• Properdin factor B

Background

Factor B is a component of the alternative pathway of complement activation. The protein consists of a single polypeptide chain of molecular mass 93 kDa, and has a concentration in plasma of approximately 200 ug/ml. Factor B is cleaved by factor D into the Ba component (30 kDa) and the Bb (63 kDa). Together with C3b, Bb forms the alternative pathway C3 convertase C3bBb

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFB