Complement Component C5aR1 Antibody (347214) [Fluorescein]

Human peripheral blood granulocytes were stained with Mouse Anti-Human Complement Component C5a R1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3648F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 347214
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Complement Component C5aR1 Antibody (347214) [Fluorescein] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Complement Component C5a R1
  Met1-Val350
  Accession # P21730
• Specificity: Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: C5AR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5aR1 Antibody (347214) [Fluorescein]

• C5a anaphylatoxin chemotactic receptor
• C5A
• C5aR
• C5a-R
• C5AR1
• C5ARC5a anaphylatoxin receptor
• C5R1C5a ligand
• CD88 antigen
• CD88
• complement component 5 receptor 1 (C5a ligand)
• complement component 5 receptor 1
• complement component 5a receptor 1
• Complement Component C5a R1
• Complement Component C5aR1

Background

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Component C5aR1 Antibody (FAB3648F)(1)

Publication using FAB3648F

• Reis E, Chen H, Sfyroera G, Monk P, Kohl J, Ricklin D, Lambris J C5a receptor-dependent cell activation by physiological concentrations of desarginated c5a: insights from a novel label-free cellular assay. J Immunol, 2012-10-05;189(10):4797-805. 2012-10-05 [PMID: 23041570] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: C5AR1
• Uniprot:
  • P21730()