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VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
Species: Hu Applications: Flow, IHC, CyTOF-ready, ICC/IF Host: Mouse Monoclonal
2037-C5) induces N-acetyl-beta -D-glucosa-minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB3648). The ND50 is typically 2-10 µg/mL in the presence of cytochalasin-B." class="big_lightbox" target="_blank">
The human anaphylatoxin C5a is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.
![Flow Cytometry Complement Component C5aR1 Antibody (347234) [Unconjugated]](http://images.novusbio.com/images/antibody/Complement_Component_C5aR1_MAB36481_Flow_Cytometry_23810.jpg)
![Neutralization Complement Component C5aR1 Antibody (347214) [Unconjugated]](http://images.novusbio.com/images/antibody/Complement_Component_C5a_R1_MAB3648_Block_Neutralize_9166.jpg)
![Immunocytochemistry Complement Component C5aR1 Antibody (347234) [Unconjugated]](http://images.novusbio.com/images/antibody/Complement_Component_C5aR1_MAB36481_Immunocytochemistry__Immunofluorescence_23921.jpg)
![Immunohistochemistry Complement Component C5aR1 Antibody (347234) [Unconjugated]](http://images.novusbio.com/images/antibody/Complement_Component_C5aR1_MAB36481_Immunohistochemistry_23922.jpg)
