Complement Component C1qA Antibody (JL-1) [Biotin]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IA, ICC/IF, IHC, IHC-Fr
Clone
JL-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

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Catalog# & Conjugate Size Price

Complement Component C1qA Antibody (JL-1) [Biotin] Summary

Immunogen
Purified mouse C1QA
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
C1QA
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Readout System

Reactivity Notes

Human and Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered biotinylated antibody solution in PBS and 0.1% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Positive control: Spleen and kidney tissue of wild-type mice (Ref.1) Negative control: Spleen and kidney tissue of C1q -/- mice (Ref.1)

Alternate Names for Complement Component C1qA Antibody (JL-1) [Biotin]

  • C1QA complement component 1, q subcomponent, A chain
  • C1QA
  • Complement Component C1qA

Background

The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q, a 459 kDa molecule consisting of three individual polypeptide chains. The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can also recognize molecular patterns associated with pathogens and it can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE). The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for Complement Component C1qA Antibody (NBP1-51140B)

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Bioinformatics

Gene Symbol C1QA
Entrez