Complement C5 Antibody (561) - Azide Free

• Reactivity: Hu
• Applications: ELISA, Func
• Clone: 561
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

Complement C5 Antibody (561) - Azide Free Summary

• Description: Novus Biologicals Mouse Complement C5 Antibody (561) - Azide Free (NBP1-60147) is a monoclonal antibody validated for use in ELISA. Anti-Complement C5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human C5/C5a
• Specificity: Monoclonal antibody 561 recognizes the N-terminus of C5. The antibody reacts both with intact C5 (190 kDa) as with C5a (115 kDa).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: C5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA detection
  • Functional
  • Immunoassay
• Application Notes: This antibody works in Functional, Immunoassay. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of C5/C5a to be inactivated.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement C5 Antibody (561) - Azide Free

• anaphylatoxin C5a analog
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• C5
• complement C5
• complement component 5
• CPAMD4MGC142298
• FLJ17816
• FLJ17822

Background

The complement system is composed of over 30 proteins, activated in response to tissue injury, invading pathogens or other foreign surfaces. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b, resulting in activation of the lytic pahway C5a was first described as a cleavage product of C5 with chemotactic and anaphylatoxic properties. Further characterization revealed that C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. Complement fragment C5a is a 74 residue pro-inflammatory polypeptide. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The former is a well-established receptor that initiates G-protein-coupled signaling via mitogen-activated protein kinase pathways, thereby by inducing synthesis of cytokines such as TNF-alpha, IL-1beta, IL-6 and IL-8. Its in vivo blockade greatly reduces inflammatory injury. Much less is known about C5L2, occupancy of which by C5a does not initiate increased intracellular Ca(2+).The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. Nowadays C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and haematopoiesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement C5 Antibody (NBP1-60147)(2)

Publications using NBP1-60147

• ung M, Lu M, Fure H, Sun W, Sun C, Shi NY, Dou Y, Su J, Swanson X, Mollnes TE. Pre-neutralization of C5a-mediated effects by the monoclonal antibody 137-26 reacting with the C5a moiety of native C5 without preventing C5 cleavage. FSource Tanox Inc., Houston, Texas 77025, USA. mfung@tanox.com . Clin Exp Immunol. 133(2):160-9. 2003-08-01 [PMID: 12869020]
• Mollnes TE, Brekke OL, Fung M, Fure H, Christiansen D, Bergseth G, Videm V, Lappegard KT, Kohl J, Lambris JD. Essential role of the C5a receptor in E coli-induced oxidative burst and phagocytosis revealed by a novel lepirudin-based human whole blood model of inflammation. Source Institute of Immunology, The National Hospital, University of Oslo, Norway. t.e.mollnes@labmed.uio.no . Blood. 100(5):1869-77. 2002-09-01 [PMID: 12176911]

FAQs for Complement C5 Antibody (NBP1-60147). (Showing 1 - 1 of 1 FAQs).

1. I am interested in purchasing the following antibody: catalog # NBP1-60147 Complement C5 Antibody. In which epitope its been raised against? What volume do you get out of 0.05mg? Also, what is its cost?
   • For NBP1-60147, Monoclonal antibody 561 recognizes the N-terminus of C5. The antibody reacts both with intact C5 (190 kDa) as with C5a (115 kDa). and the concentration is 0.1 mg/mL which is a volume of 500 uL. Regarding the price of this product, please use our website to select your country, which will allow you to view the country specific pricing, availability and technical support information.

Bioinformatics

• Gene Symbol: C5
• Entrez:
  • 727(Human)
• Uniprot:
  • P01031()