Complement C5 Antibody (561)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Func, IA
Clone
561
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Complement C5 Antibody (561) Summary

Immunogen
Human C5/C5a
Specificity
Monoclonal antibody 561 recognizes the N-terminus of C5. The antibody reacts both with intact C5 (190 kDa) as with C5a (115 kDa).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
C5
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA detection
  • Functional
  • Immunoassay
Application Notes
This antibody works in Functional, Immunoassay. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of C5/C5a to be inactivated.
Publications
Read Publications using NBP1-60147.

Reactivity Notes

This product is reactive in Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Complement C5 Antibody (561)

  • anaphylatoxin C5a analog
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
  • C5
  • complement C5
  • complement component 5
  • CPAMD4MGC142298
  • FLJ17816
  • FLJ17822

Background

The complement system is composed of over 30 proteins, activated in response to tissue injury, invading pathogens or other foreign surfaces. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b, resulting in activation of the lytic pahway C5a was first described as a cleavage product of C5 with chemotactic and anaphylatoxic properties. Further characterization revealed that C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. Complement fragment C5a is a 74 residue pro-inflammatory polypeptide. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The former is a well-established receptor that initiates G-protein-coupled signaling via mitogen-activated protein kinase pathways, thereby by inducing synthesis of cytokines such as TNF-alpha, IL-1beta, IL-6 and IL-8. Its in vivo blockade greatly reduces inflammatory injury. Much less is known about C5L2, occupancy of which by C5a does not initiate increased intracellular Ca(2+).The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. Nowadays C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and haematopoiesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Complement C5 Antibody (NBP1-60147)(2)

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Product General Protocols

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FAQs for Complement C5 Antibody (NBP1-60147). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in purchasing the following antibody: catalog # NBP1-60147 Complement C5 Antibody. In which epitope its been raised against? What volume do you get out of 0.05mg? Also, what is its cost?
    • For NBP1-60147, Monoclonal antibody 561 recognizes the N-terminus of C5. The antibody reacts both with intact C5 (190 kDa) as with C5a (115 kDa). and the concentration is 0.1 mg/mL which is a volume of 500 uL. Regarding the price of this product, please use our website to select your country, which will allow you to view the country specific pricing, availability and technical support information.

Secondary Antibodies

 

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Blogs on Complement C5.

Complement C3 - the most important protein in the complement system
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

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Bioinformatics

Gene Symbol C5
Entrez
Uniprot