Complement C5 Antibody (12F3) [DyLight 405]

Product Discontinued

Complement C5 Antibody (12F3) [DyLight 405] Summary

• Immunogen: C5 isolated from human plasma adsorbed onto aluminum hydroxide gel
• Epitope: Epitope specificity differs from that of Clone 03 but slightly overlap as determined by inhibition ELISA.
• Specificity: Clone 12F3 is specific for human C5. No reaction is seen with plasma from patients deficient in C5.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Complement C5 Antibody (12F3) [DyLight 405]

• anaphylatoxin C5a analog
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• C5
• complement C5
• complement component 5
• CPAMD4MGC142298
• FLJ17816
• FLJ17822

Background

C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C5