Complement C4d Antibody (C4D204) [DyLight 405]


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Product Details

Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Please see the vial label for concentration. If unlisted please contact technical services.

Complement C4d Antibody (C4D204) [DyLight 405] Summary

Recombinant human Complement 4d protein
Intracytoplasmic vacuoles of endothelial cells and Secreted
Acute Humoral Rejection Marker
This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
IgG1 Kappa
Protein A purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 405
DyLight 405 (A=400, E=420)
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:200-1:400
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Complement C4d Antibody (NBP2-34581V) (0)

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Secondary Antibodies


Isotype Controls

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Complement C4d NBP2-34581V

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Contact Information

Product PDFs


Gene Symbol C4A

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