Complement C4d Antibody (7H4)

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Product Details

Summary
Reactivity Hu, GpSpecies Glossary
Applications WB, ELISA, IHC
Clone
7H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Complement C4d Antibody (7H4) Summary

Immunogen
The monoclonal antibody 7H4 (also known as clone M4d2) recognizes an epitope on the alpha 2 chain of human C4d.
Specificity
The monoclonal antibody 7H4 recognizes C4, C4b and C4d.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
C4A
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunoassay
  • Immunohistochemistry-Frozen
  • Western Blot
Application Notes
For Immunohistochemistry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
0.2 um filtered solution in PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for Complement C4d Antibody (7H4)

  • C4
  • C4A
  • C4A2
  • C4A3
  • C4A4
  • C4A6
  • C4S
  • CO4
  • complement C4A
  • CPAMD2
  • RG

Background

The monoclonal antibody 7H4 (also known as clone M4d2) recognizes an epitope on the alpha 2 chain of human C4d. Complement factor C4 (MW 41 kDa), formerly known as Gg protein, consists of an alpha-, beta- and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved by factor I resulting in C4c and C4d, in which step all functional sites are lost. The C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. The thioester formed between the side chains of Cys1010 and Gln1013 within the C4d region of the alpha-chain mediates covalent attachment to the target surface bearing activated forms of C1s or MASP. Furthermore, C4d is highly homologous to C3d with over 35% shared amino acid sequence. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies. The monoclonal antibody 7H4 recognizes C4, C4b and C4d. Antibody 7H4 cross reacts with guinea pig.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP3-11291

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Bioinformatics

Gene Symbol C4A