Complement C3d Antibody (C3D/2891) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: ELISA, IHC, MA
• Clone: C3D/2891
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Complement C3d Antibody (C3D/2891) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Recombinant fragment (around aa 1250-1450) of human Complement C3d protein (exact sequence is proprietary) (Uniprot: P01024)
• Localization: Plasma.
• Marker: Acute Humoral Rejection Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin
  • Protein Array
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Complement C3d Antibody (C3D/2891) [CoraFluor™ 1]

• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• complement C3
• complement component 3
• complement component C3
• CPAMD1acylation-stimulating protein cleavage product

Background

C3d is a fragment of the major complement protein C3, C3d is formed as a result of the deactivation of C3b.Factor I in the presence of cofactors cleaves C3b to form iC3b and C3f, Factor I and/or other proteasesthen cleaves iC3b to form C3c and C3dg. C3dg is then further cleaved by tryptic enzymes to C3g andC3d. The C3 fragments are then eliminated from circulation by phagocytic cells in the liver. C3d has amolecular weight of 34kDa. Normally C3d is present in trace amounts in plasma, however, in diseasestates where rapid complement consumption is occurring levels of C3d can be elevated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3