Collagen VI alpha 1 Native Protein

SDS-Page: Collagen VI alpha 1 Native Protein [NBP1-97270] - SDS-PAGE results of Collagen VI alpha 1 Native Protein. Lane: 1) Opal prestain molecular weight marker, 2) blank, 3) Collagen VI alpha 1 Native Protein [10µg]. Coomassie stained.

Western Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] - Western blot results using Collagen VI alpha 1 Native Protein. Lane: 1) Human Collagen Type VI reduced and boiled, 3) Human Collagen Type VI reduced and not boiled,5) Opal pre-stained molecular weight marker, 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10µg. Primary Antibody: Collagen VI alpha 1 Native Protein 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer at RT for 1hr.

Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] - Collagen VI alpha 1 Native Protein. Sample: A) Human Collagen VI, B) Bovine/Human Collagen VI.Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.Primary Antibody: Rabbit Anti-Collagen VI antibody at 5µg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer at RT for 30mins.

• Reactivity: Hu
• Applications: WB, ELISA, IHC

Collagen VI alpha 1 Native Protein Summary

• Description: Native Human Collagen VI alpha 1
  
  Source: Human placenta (Accession #:P12109)
  
  Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
• Preparation Method: Prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.
• Protein/Peptide Type: Native Protein
• Gene: COL6A1
• Purity: Chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1:1000
• Application Notes: This product has been tested in SDS-Page and is used as a control in ELISA and western blot. Collagen type VI is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.5 M Acetic Acid
• Preservative: 0.01% Sodium Azide
• Purity: Chromatography

Alternate Names for Collagen VI alpha 1 Native Protein

• Collagen 6
• collagen alpha-1(VI) chain
• collagen VI, alpha-1 polypeptide
• collagen, type VI, alpha 1
• Collagen-6
• human mRNA for collagen VI alpha-1 C-terminal globular domain10alpha 1 (VI) chain (61 AA)
• OPLL

Background

Collagen type VI is a form of collagen primarily associated with the extracellular matrix of skeletal muscle. Collagen type VI is associated with the genes COL6A1, COL6A2, and COL6A3. Defects in collagen VI are associated with Bethlem myopathy and Ullrich congenital muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Collagen VI alpha 1 Native Protein (NBP1-97270)(1)

Publication using NBP1-97270

• Sano T, Ochiai T, Nagayama T et al. Genetic reduction of insulin signaling mitigates amyloid-? deposition by promoting expression of extracellular matrix proteins in the brain The Journal of neuroscience : the official journal of the Society for Neuroscience 2023-09-11 [PMID: 37699718]

Bioinformatics

• Gene Symbol: COL6A1
• Uniprot:
  • P12109(Human)