Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free

Collagen IV was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha 1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Collagen IV was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha 1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Collagen IV was detected in immersion fixed paraffin-embedded sections of human Kidney using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha 1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Western Blot: Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free [NBP3-25416] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free [NBP3-25416] - COL4A1 antibody [HL1351] detects COL4A1 protein at vesicle by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free [NBP3-25416] - COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free [NBP3-25416] - COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free [NBP3-25416] - COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, mIF
• Clone: HL1351
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus of human Collagen IV alpha 1. The exact sequence is proprietary.
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: COL4A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Multiplex Immunofluorescence 1ug/mL
  • Western Blot 1:500-1:3000
• Theoretical MW: 161 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (82%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free

• alpha-1 chain
• Arresten
• COL4A1 NC1 domain
• COL4A1
• collagen alpha-1(IV) chain
• Collagen IV alpha 1
• collagen IV, alpha-1 polypeptide
• collagen, type IV, alpha 1
• Collagen-4
• EC 3.4.23
• EC 6.3.1.2

Background

Collagen IV is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL4A1