Collagen III alpha 1/COL3A1 Native Protein

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Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Lane 1: Human Collagen III Load: 100 ug per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C Secondary antibody: DyLight (TM) 649 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Concentration
1.0 mg/ml

Order Details

Collagen III alpha 1/COL3A1 Native Protein Summary

Description
Native COL3A1 protein.

Source: Human Placenta/i>

(Accession #: P02461)

Preparation
Method
Human Collagen type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type III. Reaction with Rockland's anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Protein/Peptide Type
Native Protein
Gene
COL3A1
Purity
SDS-PAGE

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
Application Notes
Collagen type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.5 M Acetic Acid
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
SDS-PAGE

Alternate Names for Collagen III alpha 1/COL3A1 Native Protein

  • alpha1 (III) collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • EDS4A
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534

Background

Human Collagen type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type III. Reaction with Rockland's anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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WB Video Protocol

FAQs for Collagen III alpha 1/COL3A1 Native Protein (NBP1-97267). (Showing 1 - 1 of 1 FAQ).

  1. I'm interested in using colagen III in adhesion assays of cell lines to collagen-coated TC plates. I've seen you have human recombinant collagen III (H00001281-P01) but in the datasheet it says it is not suitable for functional assays. I was wondering, though, if it is OK to use it in adhesion assays. You also have non-recombinant collagen III (NBP1-97267), which I guess it's purified. Is this one suitable for adhesion assays?
    • H00001281-P01 product is distributed by us through a Taiwanese company called Abnova. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots as a positive control with their antibody. NBP1-97267 is a native protein purified from a human source.

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Bioinformatics

Gene Symbol COL3A1
Entrez