Collagen III alpha 1/COL3A1 Antibody [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Janelia Fluor 646

Collagen III alpha 1/COL3A1 Antibody [Janelia Fluor® 646] Summary

Immunogen
This Collagen III alpha 1/COL3A1 Antibody was developed by hyperimmunizing goats with human type III collagen.
Specificity
Reacts with conformational determinants on human type III collagen as demonstrated by ELISA. May react with type III collagen from other species. Exhibits <10% cross reactivity with collagen type I, II, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COL3A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Collagen III alpha 1/COL3A1 Antibody [Janelia Fluor® 646]

  • alpha1 (III) collagen
  • alpha-1 type III collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • collagenIII
  • Collagen-III
  • EDS4A
  • EDSVASC
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534
  • PMGEDSV

Background

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-26547AF350
Alexa Fluor 405 NBP1-26547AF405
Alexa Fluor 488 NBP1-26547AF488
Alexa Fluor 532 NBP1-26547AF532
Alexa Fluor 594 NBP1-26547AF594
Alexa Fluor 647 NBP1-26547AF647
Alexa Fluor 700 NBP1-26547AF700
Alexa Fluor 750 NBP1-26547AF750
Biotin NBP1-26548
Biotin NBP1-26547B
DyLight 350 NBP1-26547UV
DyLight 405 NBP1-26547V
DyLight 488 NBP1-26547G
DyLight 550 NBP1-26547R
DyLight 594 NBP1-26547DL594
DyLight 650 NBP1-26547C
DyLight 680 NBP1-26547FR
DyLight 755 NBP1-26547IR
FITC NBP1-26547F
HRP NBP1-26547H
Janelia Fluor 549 NBP1-26547JF549
Janelia Fluor 646 NBP1-26547JF646

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Bioinformatics

Gene Symbol COL3A1