Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free (NBP1-05119)

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Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) [NBP1-05119] - Human bronchus tissue

Product Details

Summary
Reactivity Hu, Rt, Ca, Kg, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
1E7-D7/Col3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free Summary

Immunogen
Acid-digested pepsin soluble human type III collagen
Epitope
Epitope is located approximately 100nm from the N-terminal of the molecule
Specificity
Highly specific for type III collagen. It has been shown to have no cross reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL3A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:800
  • Immunocytochemistry/Immunofluorescence 1:100-1:300
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
NBP1-05119 can be used for detection of collagens by ELISA. NBP1-05119 has been used successfully for immunohistology on frozen unfixed sections of human and kangaroo skin, and of new dog tissue associated with biomaterial implants. If fixation of tissue is required, acetone or ethanol is recommended.
Publications
Read Publications using
NBP1-05119 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 26373929).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free

  • alpha1 (III) collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • EDS4A
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534

Background

Type III collagen is present in the walls of arteries, intestines and the uterus, where it strengthens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen III alpha 1/COL3A1 Antibody (NBP1-05119)(7)

We have publications tested in 4 confirmed species: Human, Rat, Canine, Rabbit.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(3)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Rat
(1)
Canine
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-05119 Applications Species
Su L, Li X, Wu X et al. Simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar. Sci Rep 2016 May 16 [PMID: 27181267]
Zhou Y, Zhang J, Wu H et al. The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries. Stem Cell Res Ther 2015 Sep 16 [PMID: 26373929] (WB, ICC/IF, Rabbit) WB, ICC/IF Rabbit
Hong SJ, Jia SX, Xie P et al. Topically Delivered Adipose Derived Stem Cells Show an Activated-Fibroblast Phenotype and Enhance Granulation Tissue Formation in Skin Wounds. PLoS One 2013 [PMID: 23383253] (IHC-P, ICC/IF, Rabbit) IHC-P, ICC/IF Rabbit
Gokce A, Yilmaz I, Bircan R et al. Synergistic Effect of TGF-beta1 And BMP-7 on Chondrogenesis and Extracellular Matrix Synthesis: An In Vitro Study Open Orthop J 2012 [PMID: 23002411] (WB, Human) WB Human
Rortveit R, Lingaas F, Bonsdorff T et al. A canine autosomal recessive model of collagen type III glomerulopathy Lab Invest 2012 Oct [PMID: 22890554] (IHC, Canine) IHC Canine
He Y, Yip SL, Cheung KK et al. The effect of monochromatic infrared energy on diabetic wound healing Int Wound J 2012 Jul 9 [PMID: 22776615] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Ceylan S, Anik I, Koc K, Kokturk S, Ceylan S, Cine N, Savli H, Sirin G, Sam B, Gazioglu N. Microsurgical anatomy of membranous layers of the pituitary gland and the expression of extracellular matrix collagenous proteins. Acta Neurochir (Wien);153(12):2435-43; discussion 2443. 2011 Dec. [PMID: 21969225] (IHC, Human) IHC Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen III alpha 1/COL3A1 Antibody (NBP1-05119). (Showing 1 - 1 of 1 FAQs).

  1. What is the expected molecular weight of Collagen III alpha 1 protein?
    • The theoretical molecular weight for Collagen III alpha 1 is 138kDa. There is a smaller isoform at 111kDa as well. Due to post translational modifications, this protein may run at a higher molecular weight than the expected weight.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL3A1
Entrez