Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free


Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Human bronchus tissue

Product Details

Reactivity Hu, Rt, Ca, Kg, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
BSA Free
1.0 mg/ml

Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free Summary

Acid-digested pepsin soluble human type III collagen
Epitope is located approximately 100nm from the N-terminal of the molecule
Highly specific for type III collagen. It has been shown to have no cross reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
IgG1 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:100-1:2000
  • ELISA 1:800
  • Immunocytochemistry/Immunofluorescence 1:100-1:300
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
NBP1-05119 can be used for detection of collagens by ELISA. NBP1-05119 has been used successfully for immunohistology on frozen unfixed sections of human and kangaroo skin, and of new dog tissue associated with biomaterial implants. If fixation of tissue is required, acetone or ethanol is recommended.
Read Publications using
NBP1-05119 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 26373929).

Packaging, Storage & Formulations

Store at 4C in the dark.
10mM Phosphate (pH 7.4) and 0.5M NaCl
15mM Sodium Azide
1.0 mg/ml
Protein A or G purified

Alternate Names for Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free

  • alpha1 (III) collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • EDS4A
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534


Type III collagen is present in the walls of arteries, intestines and the uterus, where it strengthens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collagen III alpha 1/COL3A1 Antibody (NBP1-05119)(8)

We have publications tested in 4 confirmed species: Human, Rat, Canine, Rabbit.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 8 of 8.
Publications using NBP1-05119 Applications Species
Kim SJ, Song DH, Park JW et al. Effect of Bone Marrow Aspirate Concentrate-Platelet-Rich Plasma on Tendon-Derived Stem Cells and Rotator Cuff Tendon Tear Cell Transplant. 2017 May 09 [PMID: 28105983] (ICC/IF, Human) ICC/IF Human
Su L, Li X, Wu X et al. Simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar. Sci Rep 2016 May 16 [PMID: 27181267]
Zhou Y, Zhang J, Wu H et al. The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries. Stem Cell Res Ther 2015 Sep 16 [PMID: 26373929] (WB, ICC/IF, Rabbit) WB, ICC/IF Rabbit
Hong SJ, Jia SX, Xie P et al. Topically Delivered Adipose Derived Stem Cells Show an Activated-Fibroblast Phenotype and Enhance Granulation Tissue Formation in Skin Wounds. PLoS One 2013 [PMID: 23383253] (IHC-P, ICC/IF, Rabbit) IHC-P, ICC/IF Rabbit
Gokce A, Yilmaz I, Bircan R et al. Synergistic Effect of TGF-beta1 And BMP-7 on Chondrogenesis and Extracellular Matrix Synthesis: An In Vitro Study Open Orthop J 2012 [PMID: 23002411] (WB, Human) WB Human
Rortveit R, Lingaas F, Bonsdorff T et al. A canine autosomal recessive model of collagen type III glomerulopathy Lab Invest 2012 Oct [PMID: 22890554] (IHC, Canine) IHC Canine
He Y, Yip SL, Cheung KK et al. The effect of monochromatic infrared energy on diabetic wound healing Int Wound J 2012 Jul 9 [PMID: 22776615] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Ceylan S, Anik I, Koc K, Kokturk S, Ceylan S, Cine N, Savli H, Sirin G, Sam B, Gazioglu N. Microsurgical anatomy of membranous layers of the pituitary gland and the expression of extracellular matrix collagenous proteins. Acta Neurochir (Wien);153(12):2435-43; discussion 2443. 2011 Dec. [PMID: 21969225] (IHC, Human) IHC Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Collagen III alpha 1/COL3A1 Antibody (NBP1-05119). (Showing 1 - 1 of 1 FAQs).

  1. What is the expected molecular weight of Collagen III alpha 1 protein?
    • The theoretical molecular weight for Collagen III alpha 1 is 138kDa. There is a smaller isoform at 111kDa as well. Due to post translational modifications, this protein may run at a higher molecular weight than the expected weight.

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Gene Symbol COL3A1