Collagen II Antibody (II-4C11)

Product Discontinued

Collagen II Antibody (II-4C11) Summary

• Immunogen: This clone specifically reacts with the hCL(II) from human costal cartilage and cross-reacts with rabbit, bovine and rat CL(II).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1-10 ug/ml
  • Immunohistochemistry-Paraffin 1-10 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: Immunohistochemistry on frozen or paraffin sections (1-10) ug/ml). Also suitable for western blotting. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Use in ICC/IF reported in scientific literature (PMID 27185069).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27185069)

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: 0.1M Sodium Phosphate (pH 7.0) and 0.5% BSA (protease - free)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Collagen II Antibody (II-4C11)

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen II Antibody (NBP2-33343)(18)

Publications using NBP2-33343

• Yao Y, Wang P, Li X et al. A di-self-crosslinking hyaluronan-based hydrogel combined with type I collagen to construct a biomimetic injectable cartilage-filling scaffold Acta Biomater 2020-05-17 [PMID: 32434079]
• Liu J, Yu C, Chen Y et al. Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture J Mater Chem B 2020-04-09 [PMID: 32264594]
• Shudo Y, MacArthur JW, Kunitomi Y et al. Three-Dimensional Multi-layered Microstructure using Laser Direct-Writing System Tissue Eng Part A 2020-02-21 [PMID: 32085692]
• Chen M, Feng Z, Guo W et al. PCL-MECM-Based Hydrogel Hybrid Scaffolds and Meniscal Fibrochondrocytes Promote Whole Meniscus Regeneration in a Rabbit Meniscectomy Model ACS Appl Mater Interfaces 2019-10-22 [PMID: 31596568]
• Lu J, Shen X, Sun X et al. Increased recruitment of endogenous stem cells and chondrogenic differentiation by a composite scaffold containing bone marrow homing peptide for cartilage regeneration Theranostics 2018-11-16 [PMID: 30429885] (IF/IHC, Rabbit)
• Du Y, Liu H, Yang Q et al. Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. Biomaterials. 2017-08-01 [PMID: 28528301] (IF/IHC, Rabbit)
• Jakob M. Townsend, S. Connor Dennis, Jonathan Whitlow et al. Colloidal Gels with Extracellular Matrix Particles and Growth Factors for Bone Regeneration in Critical Size Rat Calvarial Defects. The AAPS Journal. [PMID: 28138909] (IF/IHC, Rat)
• Han ME, Kim SH1, Kim HD et al. Extracellular Matrix-based Cryogels for Cartilage Tissue Engineering. Int J Biol Macromol. [PMID: 27185069] (IF/IHC, IF/IHC, Mouse)
• Kim SH, Lee HR, Yu SJ et al. Hydrogel-laden paper scaffold system for origami-based tissue engineering. Proc. Natl. Acad. Sci. U.S.A. 2015-12-15 [PMID: 26621717] (IHC-P, Rabbit)
• Jia L, Chen J, Wang Y et al. Focused Low-intensity Pulsed Ultrasound Affects Extracellular Matrix Degradation via Decreasing Chondrocyte Apoptosis and Inflammatory Mediators in a Surgically Induced Osteoarthritic Rabbit Model. Ultrasound Med Biol 2015-09-21 [PMID: 26403700] (IHC-P, Rabbit)
• Yao Q, Wei B, Liu N et al. Chondrogenic regeneration using bone marrow clots and a porous polycaprolactone-hydroxyapatite scaffold by 3D printing. Tissue Eng Part A. 2014-12-20 [PMID: 25530453] (IHC-P, Rabbit)
• Wei B, Gu Q, Li D et al. Mild degenerative changes of hip cartilage in elderly patients: an available sample representative of early osteoarthritis. Int J Clin Exp Pathol. 2014-11-17 [PMID: 25400727] (IHC-P, Human)
• Tianyuan Z, Haoyuan D, Jianwei L et al. A Smart MMP13?Responsive Injectable Hydrogel with Inflammatory Diagnostic Logic and Multiphase Therapeutic Ability to Orchestrate Cartilage Regeneration Advanced Functional Materials 2023-03-03
• Cao H, Li Z, Chen Y et al. Viscoelasticity microenvironment constructed by self-crosslinking hyaluronan hybrid hydrogels regulates chondrogenic differentiation of mesenchymal stem cells Composites Part B: Engineering 2023-08-01
• Ouyang X, Wei B, Mao F et al. Uncultured bone marrow mononuclear cells delay the dedifferentiation of unexpanded chondrocytes in pellet culture. Cell Tissue Res. 2015-03-15 [PMID: 25773454] (IHC-P, Rabbit)
• Wei B, Yao Q, Guo Y et al. Three-dimensional polycaprolactone-hydroxyapatite scaffolds combined with bone marrow cells for cartilage tissue engineering. J Biomater Appl. 2015-03-11 [PMID: 25766036] (IF/IHC, Rabbit)
• Ishimaru T, Komura M, Sugiyama M, Komura H. Slow Release of Basic Fibroblast Growth Factor (B-Fgf) Enhances Mechanical Properties of Rat Trachea J Pediatr Surg 2015-02-02 [PMID: 25638613]
• Wei B, Xu Y, Mao F et al. Composite scaffolds composed of bone marrow mesenchymal stem cell-derived extracellular matrix and marrow clots promote marrow cell retention and proliferation. J Biomed Mater Res A. 2014-11-19 [PMID: 25410417] (IHC-P, Rabbit)

Review for Collagen II Antibody (NBP2-33343) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Jakob Townsend IHC-P Rat 02/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Cartilage tissue
• Species: Rat
• Lot: FF00231

Bioinformatics

• Gene Symbol: COL2A1