Coilin Antibody (Pdelta) - BSA Free

Western Blot: Coilin Antibody (Pdelta) - BSA Free [NB600-1275] - HeLa cells nuclear extract were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-Coilin antibody produced in Mouse, Clone: p-delta. The antibody was developed using 1:1000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat

Immunocytochemistry/Immunofluorescence: Coilin Antibody (Pdelta) [NB600-1275] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-Coilin Clone: pdelta (NB600-1275). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

• Reactivity: Hu, Mu(-)
• Applications: WB, ICC/IF, MA
• Clone: Pdelta
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.5 mg/ml

Coilin Antibody (Pdelta) - BSA Free Summary

• Immunogen: Recombinant fragment (C-terminus of Human coilin, 389 amino acids).
• Epitope: Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.
• Localization: Nuclear
• Specificity: Coilin (Pdelta)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COIL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Microarray
  • Western Blot 1-2 ug/ml
• Application Notes: WB: Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Theoretical MW: ~80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Nuclear Cell Lysate
  Coilin Overexpression Lysate

Reactivity Notes

Cross-reacts with Human. Does not cross-react with Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Coilin Antibody (Pdelta) - BSA Free

• CLN80p80-coilin
• coilin p80
• coilin
• p80

Background

Coilin is encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coilin Antibody (NB600-1275)(1)

Publication using NB600-1275

• Querido E, Sfeir A, Chartrand P ALKBH5 inhibited cell proliferation and sensitized bladder cancer cells to cisplatin by m6A-CK2 alpha mediated glycolysis STAR Protoc 2020-10-01 [PMID: 33111129]

Bioinformatics

• Gene Symbol: COIL
• Entrez:
  • 8161(Human)
  • 12812(Mouse)
• Uniprot:
  • P38432(Human)