COASY Antibody (OTI3B3) - Azide and BSA Free

Western Blot: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COASY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY.

Immunocytochemistry/Immunofluorescence: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY COASY.

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Human pancreas tissue using anti-COASY mouse monoclonal antibody.

Flow Cytometry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - HEK293T cells transfected with either COASY Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-COASY antibody , and then analyzed by flow cytometry.

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody.

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COASY mouse monoclonal antibody.

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-COASY mouse monoclonal antibody.

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Human breast tissue using anti-COASY mouse monoclonal antibody.

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Human Kidney tissue using anti-COASY mouse monoclonal antibody.

Immunohistochemistry: COASY Antibody (OTI3B3) - Azide and BSA Free [NBP2-72224] - Staining of paraffin-embedded Human tonsil using anti-COASY mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

COASY Antibody (OTI3B3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: COASY
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 62.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for COASY Antibody (OTI3B3) - Azide and BSA Free

• bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
• CoA synthaseUKR1
• CoASY
• Coenzyme A synthase
• DPCK
• FLJ35179
• NBPbifunctional coenzyme A synthase
• nucleotide binding protein
• phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
• pOV-2
• PPAT

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COASY