COASY Antibody (2A12)

Images

 
Immunohistochemistry-Paraffin: COASY Antibody (2A12) [H00080347-M05] - Analysis of monoclonal antibody to COASY on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
ELISA: COASY Antibody (2A12) [H00080347-M05] - Detection limit for recombinant GST tagged COASY is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
2A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

COASY Antibody (2A12) Summary

Immunogen
COASY (NP_079509, 461 a.a. ~ 564 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD
Specificity
COASY (2A12)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COASY
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Has also been used for immunohistochemistry (paraffin).

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COASY Antibody (2A12)

  • bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
  • CoA synthaseUKR1
  • CoASY
  • Coenzyme A synthase
  • DPCK
  • FLJ35179
  • NBPbifunctional coenzyme A synthase
  • nucleotide binding protein
  • phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
  • pOV-2
  • PPAT

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on COASY

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Bioinformatics

Gene Symbol COASY
Entrez
Uniprot