COASY Antibody (1H6)

Images

 
Western Blot: COASY Antibody (1H6) [H00080347-M01] - COASY monoclonal antibody (M01), clone 1H6 Analysis of COASY expression in A-431.
Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody (M01), clone 1H6.Lane 1: COASY transfected lysate(30.2 KDa).Lane 2: ...read more
Sandwich ELISA: COASY Antibody (1H6) [H00080347-M01] - Detection limit for recombinant GST tagged COASY is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

COASY Antibody (1H6) Summary

Immunogen
COASY (AAH06354, 1 a.a. ~ 249 a.a) full length recombinant protein with GST tag.MLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD
Specificity
COASY - Coenzyme A synthase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COASY
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COASY Antibody (1H6)

  • bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
  • CoA synthaseUKR1
  • CoASY
  • Coenzyme A synthase
  • DPCK
  • FLJ35179
  • NBPbifunctional coenzyme A synthase
  • nucleotide binding protein
  • phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
  • pOV-2
  • PPAT

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COASY
Entrez
Uniprot