COASY Antibody (1H6) - Azide and BSA Free

Western Blot: COASY Antibody (1H6) [H00080347-M01] - COASY monoclonal antibody (M01), clone 1H6 Analysis of COASY expression in A-431.

Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi ( Cat # H00080347-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody (M01), clone 1H6 (Cat # H00080347-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: COASY Antibody (1H6) [H00080347-M01] - Analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody (M01), clone 1H6.Lane 1: COASY transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: COASY Antibody (1H6) [H00080347-M01] - Detection limit for recombinant GST tagged COASY is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 1H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

COASY Antibody (1H6) - Azide and BSA Free Summary

• Immunogen: COASY (AAH06354, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD
• Specificity: COASY - Coenzyme A synthase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COASY
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COASY Antibody (1H6) - Azide and BSA Free

• bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
• CoA synthaseUKR1
• CoASY
• Coenzyme A synthase
• DPCK
• FLJ35179
• NBPbifunctional coenzyme A synthase
• nucleotide binding protein
• phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase
• pOV-2
• PPAT

Background

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COASY
• Entrez:
  • 80347(Human)
• Uniprot:
  • AAH06354(Human)