Coagulation Factor VII Antibody (321621)

Coagulation Factor VII was detected in immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Human Coagulation Factor VII Monoclonal Antibody (Catalog # MAB2338) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, IP, ICC/IF
• Clone: 321621
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Coagulation Factor VII Antibody (321621) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor VII
  Ala39-Pro444
  Accession # NP_062562
• Specificity: Detects human Coagulation Factor VII in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein-1, -2, -3, -4, -5, -7, -8, -9, -10, -11, -12, -13, -14, -15, -B1, rhHGFA, rhFactor X, rhFactor XI, rhThrombin, rhuPA-1, or rhPROC is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: F7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor VII Antibody (321621)

• coagulation factor VII (serum prothrombin conversion accelerator)
• Coagulation Factor VII
• EC 3.4.21
• EC 3.4.21.21
• Eptacog alfa
• F7
• FVII coagulation protein
• proconvertin
• Serum prothrombin conversion accelerator
• SPCA

Background

Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor (3, 4). After a signal peptide (aa 1 to 38), the mature chain can be further processed into the light chain (aa 39 to 190) and the heavy chain (aa 191 to 444).

1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1659. 
2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
3. Hagen, F.S, et al. (1986) Proc. Natl. Acad. Sci. USA 83:2412.
4. O’Hara, P.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5158.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor VII Antibody (MAB2338)(2)

Publications using MAB2338

• Qing Zhang, Xiu-Jun Liu, Lian Hu, Dong-Sheng Liao, Yan-Bo Zheng, Yong-Su Zhen et al. Factor VII light chain-targeted lidamycin targets tissue factor-overexpressing tumor cells for cancer therapy International Journal of Molecular Medicine 2011-11-22 [PMID: 22108624]
• Qing Zhang, Xiu-Jun Liu, Lian Hu, Dong-Sheng Liao, Yan-Bo Zheng, Yong-Su Zhen et al. Factor VII light chain-targeted lidamycin targets tissue factor-overexpressing tumor cells for cancer therapy International Journal of Molecular Medicine 2011-11-22 [PMID: 22108624] (Western Blot, ELISA Capture, Human)

Bioinformatics

• Gene Symbol: F7
• Uniprot:
  • NP_062562()