ATP5J Antibody

Images

 
Western Blot: ATP5J Antibody [NBP2-15514] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ATP5J Antibody [NBP2-15514] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514] - ATP5J antibody detects ATP5J protein at mitochondria in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. ATP5J ...read more
Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514] - Immunohistochemical analysis of paraffin-embedded J5 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514] - ATP5J antibody detects ATP5J protein at mitochondria in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ATP5J antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATP5J Antibody Summary

Immunogen
Full length human ATP5J Recombinant protein.
Localization
Mitochondrion; Mitochondrion inner membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5J
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for ATP5J Antibody

  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
  • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
  • ATP5
  • ATP5A
  • ATPase subunit F6
  • ATPM
  • coupling factor 6
  • F6
  • mitochondrial ATP synthase, coupling factor 6
  • mitochondrial ATP synthase, subunit F6
  • mitochondrial ATPase coupling factor 6
  • mitochondrial
  • proliferation-inducing protein 36

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATP5J
Uniprot