ATP5J Antibody - BSA Free

Western Blot: ATP5J Antibody [NBP2-15514] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa membrane 15% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ATP5J Antibody [NBP2-15514] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: ATP5J Antibody [NBP2-15514] - Immunohistochemical analysis of paraffin-embedded J5 xenograft, using antibody at 1:500 dilution.

Western Blot: ATP5J Antibody [NBP2-15514] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J antibody (NBP2-15514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP5J Antibody - BSA Free Summary

• Immunogen: Full length human ATP5J Recombinant protein.
• Localization: Mitochondrion; Mitochondrion inner membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5PF
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ATP5J Antibody - BSA Free

• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
• ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
• ATP5
• ATP5A
• ATPase subunit F6
• ATPM
• coupling factor 6
• F6
• mitochondrial ATP synthase, coupling factor 6
• mitochondrial ATP synthase, subunit F6
• mitochondrial ATPase coupling factor 6
• mitochondrial
• proliferation-inducing protein 36

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and F0 interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ATP5PF
• Uniprot:
  • P18859(Human)