Coagulation Factor III/Tissue Factor Antibody (CL3805) - Azide and BSA Free

Staining of human colon shows strong immunoreactivity in pericryptal sheath cells.

Staining of human kidney shows moderate positivity in renal glomeruli.

Staining of human cerebral cortex shows moderate positivity in neuropil.

Staining of human lung cancer shows strong immunoreactivity in a subset of cells.

Staining of human skeletal muscle shows absence of immunoreactivity (negative control).

Staining of human placenta shows strong immunoreactivity in blood vessels.

Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Coagulation Factor III/Tissue Factor antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Staining of human testis shows strong immunoreactivity in the lamina propria.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3805
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Coagulation Factor III/Tissue Factor Antibody (CL3805) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P13726, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: F3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: IHC-Paraffin HIER pH6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody (CL3805) - Azide and BSA Free

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Coagulation Factor III/Tissue Factor Antibody (NBP3-44077). (Showing 1 - 1 of 1 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3