Coagulation Factor II/Thrombin Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Coagulation Factor II/Thrombin at approximately 95 kDa (as indicated) using 10 µg/mL of Goat ...read more
Human peripheral blood monocytes were stained with Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Coagulation Factor II/Thrombin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor II/Thrombin
Specificity
Detects human Coagulation Factor II/Thrombin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) PROC is observed, and less than 1% cross-reactivity with rhFactor X, recombinant mouse (rm) Factor X and rmPROC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
F2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF1148 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor II/Thrombin Antibody [Unconjugated]

  • coagulation factor II (thrombin) receptor-like 2
  • Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
  • Coagulation factor II receptor-like 2
  • Coagulation Factor II
  • F2
  • PAR-3
  • PAR3proteinase-activated receptor 3
  • protease-activated receptor 3
  • proteinase-activated receptor-3
  • PT
  • serine protease
  • Thrombin receptor-like 2
  • Thrombin

Background

Coagulation Factor II, commonly known as thrombin, is an essential component of the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin (1). It also activates platelets and regulates the behavior of additional cells through protease‑activated receptors (PARs) (2). It may have either protective or deleterious functions, depending on the level and location (3). Its activity is regulated by endogenous inhibitors such as anti-thrombin III (serpin C1) or heparin cofactor II (serpin D1). A plasma serine protease, thrombin is synthesized in the liver as a 622 amino acid precursor with a 24 amino acid signal peptide. Cleavage by itself or by similar enzymes converts the proenzyme to three forms designated as alpha -, beta - and gamma -thrombin. Composed of a disulfide bond-linked dimer of the light chain (A) (residues 328‑363) and the heavy chain (B) (residues 364‑622), alpha -thrombin displays the diverse functions as described above. In comparison, the further processed B chains of beta - and gamma -thrombin have no known physiological function, but retain most of the activity towards small synthetic substrates (4).

  1. Degen, S.J. and E.W. Davie (1987) Biochemistry 26:6165.
  2. Coughlin, S.R. (2000) Nature 407:258.
  3. Xi, G. et al. (2003) J. Neurochem. 84:3.
  4. Rydel, T.J. et al. (1994) J. Biol. Chem. 269:22000.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Coagulation Factor II/Thrombin Antibody (AF1148)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Western Blot.


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FAQs for Coagulation Factor II/Thrombin Antibody (AF1148). (Showing 1 - 1 of 1 FAQ).

  1. Do you have neutralizing antibodies for anti-mouse Thrombin?
    • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol F2