Recombinant Human CNTF Protein

SDS-Page: Recombinant Human CNTF Protein [NBP1-99942]

Product Discontinued

Recombinant Human CNTF Protein Summary

• Description: Recombinant bioactive protein containing 200 amino acids for Human CNTF
  
  

  Source:E. coli
  Amino Acid Sequence:(P26441)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CNTF
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 22.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized without additives
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in 5 mM Sodium phosphate (pH 7.5) to a concentration of 0.1 - 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage

Alternate Names for Recombinant Human CNTF Protein

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

CNTF (Ciliary Neurotrophic Factor) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF has also been shown to promote survivability and differentiation of other neuronal cell types. Recombinant human CNTF is a 22.9 kDa protein containing 200 amino acid residues

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CNTF