Mouse CNTF ELISA Kit (Chemiluminescence)

ELISA: Mouse CNTF ELISA Kit (Chemiluminescence) [NBP2-75796] - Samples were spiked with high concentrations of Mouse CNTF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse CNTF ELISA Kit (Chemiluminescence) [NBP2-75796] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 12.50-800 pg/mL
• Sensitivity: 7.50 pg/mL

Mouse CNTF ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Mouse CNTF in samples. No significant cross-reactivity or interference between Mouse CNTF and analogues was observed.
• Standard Curve Range: 12.50-800 pg/mL
• Sensitivity: 7.50 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 8.54%
• Intra-Assay: CV% < 9.68%
• Spike Recovery: 91-112%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: CNTF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CNTF ELISA Kit (Chemiluminescence)

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CNTF ELISA Kit (NBP2-75796). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CNTF