Human CNTF ELISA Kit (Colorimetric) Summary
| Sensitivity |
0.2 ng/mL |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CNTF |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
CNTF (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CNTF. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): 30 ml of animal serum with 0.09% sodium azide as preservative. ForStandard/Sample (serum/plasma) diluent.
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
- CNTF Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human CNTF.
- Detection Antibody CNTF (Item F): 2 vial of biotinylated anti-human CNTF (each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 8 µl of 15,000x concentrated HRP-conjugated streptavidin.
- Standards (Item C): 2 vials, recombinant human CNTF.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human CNTF ELISA Kit (Colorimetric)
Background
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for CNTF ELISA Kit (KA1703). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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