Human CNTF ELISA Kit (Colorimetric)

Images

 

Product Details

Summary
Product Discontinued
View other related CNTF ELISA Kits

Order Details


    • Catalog Number
      KA1703
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Human CNTF ELISA Kit (Colorimetric) Summary

Sensitivity
0.2 ng/mL
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CNTF

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
CNTF (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CNTF.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): 30 ml of animal serum with 0.09% sodium azide as preservative. ForStandard/Sample (serum/plasma) diluent.
  2. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
  3. CNTF Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human CNTF.
  4. Detection Antibody CNTF (Item F): 2 vial of biotinylated anti-human CNTF (each vial is enough to assay half microplate).
  5. HRP-Streptavidin Concentrate (Item G): 8 µl of 15,000x concentrated HRP-conjugated streptavidin.
  6. Standards (Item C): 2 vials, recombinant human CNTF.
  7. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human CNTF ELISA Kit (Colorimetric)

  • ciliary neurotrophic factor
  • CNTF
  • HCNTF

Background

CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

7734-LF
Species: Hu
Applications: BA
DBD00
Species: Hu
Applications: ELISA
M6000B
Species: Mu
Applications: ELISA
267-N3
Species: Hu
Applications: BA
DGP00
Species: Hu
Applications: ELISA
233-FB
Species: Hu
Applications: BA
AF-303-NA
Species: Hu
Applications: Neut, WB
8475-OM/CF
Species: Hu
Applications: BA
NBP1-83349
Species: Hu
Applications: ICC/IF, IHC,  IHC-P, KD, WB
212-GD
Species: Hu
Applications: Bind, BA
256-GF
Species: Hu
Applications: BA
AF-249-NA
Species: Hu
Applications: Neut, WB
MAB1799
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr,  IHC-P, Simple Western, WB
D1100
Species: Hu
Applications: ELISA
612-CD
Species: Hu
Applications: BA
268-N4
Species: Hu
Applications: BA
NBP1-30052
Species: Ch, Gp, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr,  IHC-P, WB

Publications for CNTF ELISA Kit (KA1703) (0)

There are no publications for CNTF ELISA Kit (KA1703).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CNTF ELISA Kit (KA1703) (0)

There are no reviews for CNTF ELISA Kit (KA1703). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CNTF ELISA Kit (KA1703). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Additional CNTF Products

Research Areas for CNTF ELISA Kit (KA1703)

Find related products by research area.

Blogs on CNTF

There are no specific blogs for CNTF, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Human CNTF ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol CNTF