CNTF Antibody (34738) [Alexa Fluor® 488]

• Reactivity: Rt
• Applications: B/N, ELISA(Cap), ELISA(Det)
• Clone: 34738
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CNTF Antibody (34738) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant rat CNTF
  Ala2-Met200
  Accession # P20294.1
• Specificity: Detects rat CNTF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human CNTF is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNTF Antibody (34738) [Alexa Fluor® 488]

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.