CLEC4F/CLECSF13 Antibody [Unconjugated]

CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse CLEC4F/CLECSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CLEC4F/CLECSF13 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
  Ala65-Gly548
  Accession # P70194
• Specificity: Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLEC4F
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC4F/CLECSF13 Antibody [Unconjugated]

• CLEC4F
• CLECSF13
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13
• C-type lectin 13
• C-type lectin domain family 4, member F
• Galactose Particle Receptor
• KCLR
• KCR

Background

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
4. Accession # P70194.
5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC4F/CLECSF13 Antibody (AF2784)(25)

Publications using AF2784

• Zhao, D;Huang, Z;Li, X;Wang, H;Hou, Q;Wang, Y;Yan, F;Yang, W;Liu, D;Yi, S;Han, C;Hao, Y;Tang, L; GDF2 and BMP10 coordinate liver cellular crosstalk to maintain liver health eLife 2024-10-25 [PMID: 39453386] (Immunohistochemistry, Transgenic Mouse)
• Halimani, N;Nesterchuk, M;Tsitrina, AA;Sabirov, M;Andreichenko, IN;Dashenkova, NO;Petrova, E;Kulikov, AM;Zatsepin, TS;Romanov, RA;Mikaelyan, AS;Kotelevtsev, YV; Knockdown of Hyaluronan synthase 2 suppresses liver fibrosis in mice via induction of transcriptomic changes similar to 4MU treatment Scientific reports 2024-02-02 [PMID: 38307876] (IHC, Mouse)
• Katoch, O;Ungalara, R;Kaminski, T;Li, Z;Dubey, RK;Burholt, I;Gudapati, S;Pradhan-Sundd, T; Long-Term L-Glutamine Treatment Reduces Hemolysis without Ameliorating Hepatic Vaso-Occlusion and Liver Fibrosis in a Mouse Model of Sickle Cell Disease Biomedicines 2023-08-29 [PMID: 37760853] (IHC, Mouse)
• Zheng L, Ling W, Zhu D et al. Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles iScience 8/1/2023 [PMID: 37554446]
• Andrea D. Kim, Lin Kui, Benedikt Kaufmann, Sung Eun Kim, Aleksandra Leszczynska, Ariel E. Feldstein Myeloid-specific deletion of chitinase-3-like 1 protein ameliorates murine diet-induced steatohepatitis progression Journal of Molecular Medicine 7/1/2023 [PMID: 37166517]
• L Li, L Cui, P Lin, Z Liu, S Bao, X Ma, H Nan, W Zhu, J Cen, Y Mao, X Ma, L Jiang, Y Nie, F Ginhoux, Y Li, H Li, L Hui Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancers Cell Stem Cell, 2023-02-13;0(0):. 2023-02-13 [PMID: 36787740] (IHC, Mouse)
• NA Trzaskalsk, B Vulesevic, MA Nguyen, N Jeraj, E Fadzeyeva, NM Morrow, CA Locatelli, N Travis, AA Hanson, JR Nunes, C O'Dwyer, JN Van der Ve, I Lorenzen-S, R Seymour, SM Pulente, AC Clément, AM Crawley, RL Jacobs, MA Doyle, CL Cooper, KH Kim, MD Fullerton, EE Mulvihill Hepatocyte-derived DPP4 regulates portal GLP-1 bioactivity, glucose production, and its absence alters liver disease progression JCI Insight, 2023-01-24;0(0):. 2023-01-24 [PMID: 36472923] (ICC/IF, Mouse)
• RE Mainz, S Albers, M Haque, R Sonntag, NS Treichel, T Clavel, E Latz, KM Schneider, C Trautwein, T Otto NLRP6 Inflammasome Modulates Disease Progression in a Chronic-Plus-Binge Mouse Model of Alcoholic Liver Disease Cells, 2022-01-06;11(2):. 2022-01-06 [PMID: 35053298] (IHC, Mouse)
• Guilliams M, Bonnardel J, Haest B Et al. Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches Cell 1/12/2022 [PMID: 35021063]
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• K Cohen, O Mouhadeb, S Ben Shlomo, M Langer, A Neumann, N Erez, I Moshkovits, R Pelet, DJ Kedar, E Brazowski, M Guilliams, HS Goodridge, N Gluck, C Varol COMMD10 is critical for Kupffer cell survival and controls Ly6Chi monocyte differentiation and inflammation in the injured liver Cell Reports, 2021-11-16;37(7):110026. 2021-11-16 [PMID: 34788631] (IHC, Mouse)
• Xinyu Yang, Di Lu, Rui Wang, Zhengxing Lian, Zuyuan Lin, Jianyong Zhuo et al. Single‐cell profiling reveals distinct immune phenotypes that contribute to ischaemia‐reperfusion injury after steatotic liver transplantation Cell Proliferation 10/1/2021 [PMID: 34469018]
• Han Y, Onufer E, Huang L et al. Enterically derived high-density lipoprotein restrains liver injury through the portal vein Science 7/23/2021 [PMID: 34437091]
• T Ishikiriya, H Nakashima, K Endo-Umeda, M Nakashima, S Ito, M Kinoshita, M Ikarashi, M Makishima, S Seki Contrasting functional responses of resident Kupffer cells and recruited liver macrophages to irradiation and liver X receptor stimulation PLoS ONE, 2021-07-23;16(7):e0254886. 2021-07-23 [PMID: 34297734] (Flow Cytometry, Mouse)
• Sabine Daemen, Mandy M. Chan, Joel D. Schilling Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH STAR Protocols 6/1/2021 [PMID: 33997821]
• Y Jiang, Y Tang, C Hoover, Y Kondo, D Huang, D Restagno, B Shao, L Gao, J Michael Mc, M Zhou, R Silasi-Man, S McGee, M Jiang, X Bai, F Lupu, C Ruan, JD Marth, D Wu, Y Han, L Xia Kupffer cell receptor CLEC4F is important for the destruction of desialylated platelets in mice Cell Death and Differentiation, 2021-05-15;0(0):. 2021-05-15 [PMID: 33993195] (IHC, Western Blot, Mouse)
• Alexandra A Tsitrina, Igor V Krasylov, Dmitry I Maltsev, Irina N Andreichenko, Viktoria S Moskvina, Dmitry N Ivankov et al. Inhibition of hyaluronan secretion by novel coumarin compounds and chitin synthesis inhibitors Glycobiology 9/9/2021 [PMID: 33978736]
• Darrell Pilling, Tejas R. Karhadkar, Richard H. Gomer A CD209 ligand and a sialidase inhibitor differentially modulate adipose tissue and liver macrophage populations and steatosis in mice on the Methionine and Choline-Deficient (MCD) diet PLOS ONE 12/30/2020 [PMID: 33378413]
• Anneleen Remmerie, Liesbet Martens, Tinne Thoné, Angela Castoldi, Ruth Seurinck, Benjamin Pavie et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver Immunity 9/15/2020 [PMID: 32888418]
• Qi Zhao, Maria del Pilar Molina-Portela, Asma Parveen, Alexander Adler, Christina Adler, Hock E et al. Heterogeneity and chimerism of endothelial cells revealed by single-cell transcriptome in orthotopic liver tumors Angiogenesis 11/1/2020 [PMID: 32440964]
• L Verboom, A Martens, D Priem, E Hoste, M Sze, H Vikkula, L Van Hove, S Voet, J Roels, J Maelfait, L Bongiovann, A de Bruin, CL Scott, Y Saeys, M Pasparakis, MJM Bertrand, G van Loo OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis Cell Rep, 2020-02-18;30(7):2237-2247.e6. 2020-02-18 [PMID: 32075762] (Flow Cytometry, Mouse)
• Friedrich Felix Hoyer, Kamila Naxerova, Maximilian J. Schloss, Maarten Hulsmans, Anil V. Nair, Partha Dutta et al. Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge Immunity 11/12/2019 [PMID: 31732166]
• Bonnardel J, T'Jonck W, Gaublomme D et al.. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche Immunity. 10/15/2019 [PMID: 31561945]
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
• C Hage, S Hoves, M Ashoff, V Schandl, S Hört, N Rieder, C Heichinger, M Berrera, CH Ries, F Kiessling, T Pöschinger Characterizing responsive and refractory orthotopic mouse models of hepatocellular carcinoma in cancer immunotherapy PLoS ONE, 2019-07-10;14(7):e0219517. 2019-07-10 [PMID: 31291357] (IHC-P, Mouse)
• Charlotte L. Scott, Wouter T’Jonck, Liesbet Martens, Helena Todorov, Dorine Sichien, Bieke Soen et al. The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages Immunity 8/21/2018 [PMID: 30076102]
• KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice Sci Rep, 2018-05-01;8(1):6789. 2018-05-01 [PMID: 29717162] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CLEC4F
• Uniprot:
  • P70194()