CLEC4F/CLECSF13 Antibody (370901) [Unconjugated]

CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse CLEC4F/CLECSF13 Monoclonal Antibody (Catalog # MAB2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

CETP protein is not co‐localized with Ly6C protein, but does co‐localize with Clec4f protein. Livers of non‐injected female APOE*3‐Leiden‐CETP mice were assayed for co‐localization of CETP and F4/80 (A), Ly6C (B) and Clec4f (C). Red; F4/80, Ly6C or Clec4f, Green; CETP, Blue; DAPI. Double headed arrows indicate co‐localization, single‐headed arrows indicate no co‐localization. CETP indicates cholesteryl ester transfer protein; Clec4f, C‐type lectin domain family 4; Ly6C, lymphocyte antigen 6 complex locus C. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29525783), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 370901
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CLEC4F/CLECSF13 Antibody (370901) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
  Ala65-Gly548
  Accession # P70194
• Specificity: Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF4F, rhCLEC4D, rhCLEC4E, recombinant mouse (rm) OCIL, and rmOCIL-rp2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CLEC4F
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC4F/CLECSF13 Antibody (370901) [Unconjugated]

• CLEC4F
• CLECSF13
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13
• C-type lectin 13
• C-type lectin domain family 4, member F
• Galactose Particle Receptor
• KCLR
• KCR

Background

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
4. Accession # P70194.
5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC4F/CLECSF13 Antibody (MAB2784)(15)

Publications using MAB2784

• Wang X, Wang Z, Liu B et al. Characteristic gene expression in the liver monocyte-macrophage-DC system is associated with the progression of fibrosis in NASH Frontiers in Immunology 2/24/2023 [PMID: 36911682]
• Wang X, Wang Z, Liu B et al. Characteristic gene expression in the liver monocyte-macrophage-DC system is associated with the progression of fibrosis in NASH Frontiers in Immunology 2023-02-24 [PMID: 36911682]
• Ragnar O. Vilmundarson, Niloufar Heydarikhorneh, An Duong, Tiffany Ho, Kianoosh Keyhanian, Fariborz Soheili et al. Savior Siblings Might Rescue Fetal Lethality But Not Adult Lymphoma in Irf2bp2-Null Mice Frontiers in Immunology 7/4/2022 [PMID: 35865523]
• Guilliams M, Bonnardel J, Haest B Et al. Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches Cell 1/12/2022 [PMID: 35021063]
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Y Jiang, Y Tang, C Hoover, Y Kondo, D Huang, D Restagno, B Shao, L Gao, J Michael Mc, M Zhou, R Silasi-Man, S McGee, M Jiang, X Bai, F Lupu, C Ruan, JD Marth, D Wu, Y Han, L Xia Kupffer cell receptor CLEC4F is important for the destruction of desialylated platelets in mice Cell Death and Differentiation, 2021-05-15;0(0):. 2021-05-15 [PMID: 33993195] (IHC, Mouse)
• Maud Voisin, Elina Shrestha, Claire Rollet, Cyrus A. Nikain, Tatjana Josefs, Mélanie Mahé et al. Inhibiting LXR alpha phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice Communications Biology 3/26/2021 [PMID: 33772096]
• Maud Voisin, Elina Shrestha, Claire Rollet, Cyrus A. Nikain, Tatjana Josefs, Mélanie Mahé et al. Inhibiting LXR alpha phosphorylation in hematopoietic cells reduces inflammation and attenuates atherosclerosis and obesity in mice Communications Biology 2021-03-26 [PMID: 33772096] (Immunohistochemistry, Mouse)
• Dániel Kucsera, Viktória E. Tóth, Dorottya Gergő, Imre Vörös, Zsófia Onódi, Anikó Görbe et al. Characterization of the CDAA Diet-Induced Non-alcoholic Steatohepatitis Model: Sex-Specific Differences in Inflammation, Fibrosis, and Cholesterol Metabolism in Middle-Aged Mice Frontiers in Physiology 2/22/2021 [PMID: 33692700]
• Anneleen Remmerie, Liesbet Martens, Tinne Thoné, Angela Castoldi, Ruth Seurinck, Benjamin Pavie et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver Immunity 9/15/2020 [PMID: 32888418]
• V Osinski, DK Bauknight, SSK Dasa, MJ Harms, T Kroon, MA Marshall, JC Garmey, AT Nguyen, J Hartman, A Upadhye, P Srikakulap, A Zhou, G O'Mahony, AL Klibanov, KA Kelly, J Boucher, CA McNamara In vivo liposomal delivery of PPARalpha/gamma dual agonist tesaglitazar in a model of obesity enriches macrophage targeting and limits liver and kidney drug effects Theranostics, 2020-01-01;10(2):585-601. 2020-01-01 [PMID: 31903139] (IHC, Mouse)
• Dustin K. Bauknight, Victoria Osinski, Siva Sai Krishna Dasa, Anh T. Nguyen, Melissa A. Marshall, Julia Hartman et al. Importance of thorough tissue and cellular level characterization of targeted drugs in the evaluation of pharmacodynamic effects PLOS ONE 11/14/2019 [PMID: 31725756]
• Seung-Jin K, Dechun F, Adrien G et al. Adipocyte Death Preferentially Induces Liver Injury and Inflammation Through the Activation of Chemokine (C-C Motif) Receptor 2-Positive Macrophages and Lipolysis Hepatology. 3/18/2019 [PMID: 30681731]
• Seung-Jin K, Dechun F, Adrien G et al. Adipocyte Death Preferentially Induces Liver Injury and Inflammation Through the Activation of Chemokine (C-C Motif) Receptor 2-Positive Macrophages and Lipolysis Hepatology. 2019-03-18 [PMID: 30681731]
• SJL van der Tu, Z Li, JFP Berbée, I Verkouter, LE Ringnalda, AE Neele, JB van Klinke, SS Rensen, J Fu, MPJ de Winther, AK Groen, PCN Rensen, K Willems va, Y Wang Lipopolysaccharide Lowers Cholesteryl Ester Transfer Protein by Activating F4/80+Clec4f+Vsig4+Ly6C- Kupffer Cell Subsets J Am Heart Assoc, 2018-03-10;7(6):. 2018-03-10 [PMID: 29525783] (IHC, Mouse)
• Itoh M, Suganami T, Kato H et al. CD11c+ resident macrophages drive hepatocyte death-triggered liver fibrosis in a murine model of nonalcoholic steatohepatitis. JCI Insight. 11/16/2017 [PMID: 29202448]

Bioinformatics

• Gene Symbol: CLEC4F
• Uniprot:
  • P70194()