CLEC4F/CLECSF13 Antibody (370901) [Alexa Fluor™ Plus 555]

• Reactivity: Mu
• Clone: 370901
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 555

CLEC4F/CLECSF13 Antibody (370901) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF4F, rhCLEC4D, rhCLEC4E, recombinant mouse (rm) OCIL, and rmOCIL-rp2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC4F/CLECSF13 Antibody (370901) [Alexa Fluor™ Plus 555]

• CLEC4F
• CLECSF13
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13
• C-type lectin 13
• C-type lectin domain family 4, member F
• Galactose Particle Receptor
• KCLR
• KCR

Background

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
4. Accession # P70194.
5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.