CLEC4E Antibody

Western Blot: CLEC4E Antibody [NBP2-27093] - Analysis of CLEC4E using NBP2-27093. Human spleen cell lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, 4) rat spleen, 5) Ramos and 6) THP1 cell lysate probed with 2 ug/ml of CLEC4E antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Pm, RM, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CLEC4E Antibody Summary

• Immunogen: This antibody was raised against CLEC4E
• Predicted Species: Rhesus Macaque (94%), Sheep (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLEC4E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4ug/ml

Reactivity Notes

Cow (75%), rat (68%), panda (74%), dog (71%), horse (75%), and African bush elephant (65%), mouse, and rabbit (67%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CLEC4E Antibody

• CLEC4E
• CLECSF9
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9
• C-type lectin domain family 4 member E
• C-type lectin domain family 4, member E
• Macrophage-inducible C-type lectin
• MINCLE
• MINCLEC-type lectin superfamily member 9

Background

A member of the C-type lectin superfamily, CLEC4E, also known as Mincle, is a ~25kDa protein. CLEC4E is expressed and found on the surface of activated macrophages. CLEC4E has also been associated with expression on innate effector cells such as dendritic cells. It serves as a receptor for pathogenic fungi such as Malassezia which causes atopic dermatitis, tinea versicolor or acute sepsis reactions. CLEC4E also serves as a sensor of damaged cells. Shown to be associated with FcRfx chain, CLEC4E also may be associated with TLR since Mincle-/- mice have above background cytokine production. CLEC4E specific antibody enables more in-depth analysis of mechanism of action, signaling pathways and development of therapies and drugs which are Mincle (CLEC4E) associated. CLEC4E is an innate immune receptor, a PAMP sensor involved in inflammatory response activation pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC4E
• Uniprot:
  • Q9ULY5()