Complexin-2 Antibody [Unconjugated]

HAF109). A specific band was detected for Complexin-2 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." > HAF109). A specific band was detected for Complexin-2 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." title="Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Complexin-2 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." />

Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the nuclei were counterstained with DAPI (blue). Complexin-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the nuclei were counterstained with DAPI (blue). Complexin-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="SH-SY5Y human neuro-blastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Complexin-2 was detected using a Goat Anti-Human/Mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the nuclei were counterstained with DAPI (blue). Complexin-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

SH-SY5Y human neuro-blastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Complexin-2 was detected using a Goat Anti-Human/Mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." > NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." title="Complexin‑2 was detected in immersion fixed L6 rat myoblast cell line and A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Complexin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." />

Complexin‑2 was detected in immersion fixed L6 rat myoblast cell line and A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Complexin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Complexin-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Complexin-2
  Met1-Lys134
  Accession # Q6PUV4
• Specificity: Detects endogenous human, mouse and rat Complexin-2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CPLX2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complexin-2 Antibody [Unconjugated]

• complexin 2
• Complexin II
• Complexin-2
• CPLX2
• CPX II
• CPX2
• CPX-2
• CPX-II
• DKFZp547D155
• Hfb1
• MGC138492
• Synaphin 1
• synaphin 1,921-L
• synaphin-1

Background

Complexin-2 (CPX II; also known as synaphin-1) is a 19 kDa, cytosolic protein that belongs to the complexin/synaphin family of molecules. It is expressed in excitatory neurons and serves as a block against premature granule release by its binding to the cis-SNARE complex that contains SNAP25, VAMP2 and STX1A. Human Complexin-2 is 134 amino acids (aa) in length and contains one coiled-coil domain (aa 28‑84) that incorporates part of a SNARE complex interaction sequence (aa 41‑97). There is one potential splice variant that shows a premature truncation after Glu114. Human Complexin-2 is identical in aa sequence to that for mouse and rat Complexin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complexin-2 Antibody (AF5085)(1)

Publication using AF5085

• Yeli Zhou, Jing Li, Feng Xu, Encheng Ji, Chenglong Wang, Zheer Pan Long noncoding RNA H19 alleviates inflammation in osteoarthritis through interactions between TP53, IL-38, and IL-36 receptor Bone & Joint Research 2022-08-17 [PMID: 35942891]

Bioinformatics

• Gene Symbol: CPLX2
• Uniprot:
  • Q6PUV4(Human)