CLEC4E Antibody (2455C) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human CLEC4e or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human CLEC4e Monoclonal Antibody (Catalog # MAB8995) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 2455C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CLEC4E Antibody (2455C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line, CHO-derived recombinant human CLEC4E
  Arg41-Leu219
  Accession # Q9ULY5
• Specificity: Detects human CLEC4E in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC4E Antibody (2455C) [Unconjugated]

• CLEC4E
• CLECSF9
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9
• C-type lectin domain family 4 member E
• C-type lectin domain family 4, member E
• Macrophage-inducible C-type lectin
• MINCLE
• MINCLEC-type lectin superfamily member 9

Background

CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Human CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, human CLEC4E shares 65% and 68% aa sequence identity with mouse and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).

1. Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
2. Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
3. Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
4. Wells, C.A. et al. (2008) J. Immunol. 180:7404.
5. Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
6. Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
7. Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
8. Ostrop, J. et al. (2015) J. Immunol. 195:2417.
9. Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
10. Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
11. Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9ULY5()