CLEC4E Antibody (2455C) [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Clone: 2455C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 680

CLEC4E Antibody (2455C) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human CLEC4E in direct ELISAs.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC4E Antibody (2455C) [Alexa Fluor™ Plus 680]

• CLEC4E
• CLECSF9
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9
• C-type lectin domain family 4 member E
• C-type lectin domain family 4, member E
• Macrophage-inducible C-type lectin
• MINCLE
• MINCLEC-type lectin superfamily member 9

Background

CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Human CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, human CLEC4E shares 65% and 68% aa sequence identity with mouse and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).

1. Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
2. Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
3. Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
4. Wells, C.A. et al. (2008) J. Immunol. 180:7404.
5. Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
6. Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
7. Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
8. Ostrop, J. et al. (2015) J. Immunol. 195:2417.
9. Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
10. Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
11. Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.