CLEC-2A Antibody (705529)

Western blot shows lysates of U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC-2A at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CLEC-2A was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 705529
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CLEC-2A Antibody (705529) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CLEC-2A
  Trp49-Leu174 (predicted)
  Accession # Q6UVW9
• Specificity: Detects human CLEC-2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC-2D, rhCLEC-2, or rhCD69 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC2A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC-2A Antibody (705529)

• CLEC2A C-type lectin domain family 2, member A
• CLEC2A
• CLEC-2A
• KACL
• PILAR
• UNQ5792

Background

CLEC-2A (C-type lectin domain family 2 member A), also known as PILAR and KACL, is an approximately 35 kDa type II transmembrane glycoprotein in the C-type lectin superfamily. It contains one C-type lectin domain in its extracellular region. Alternate splicing of human CLEC-2A generates an additional isoform with a substitution of 37 amino acids (aa) at the C-terminus. CLEC-2A is upregulated on antigen-activated CD8+ T cells and enhances T cell proliferation through interactions with CD161. It is also expressed on keratinocytes and binds to NKp65/KLRF2, triggering NK cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC2A
• Uniprot:
  • Q6UVW9(Human)