NKp80/KLRF1 Antibody [Unconjugated] - C-terminus

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NKp80/KLRF1 Antibody [Unconjugated] - C-terminus Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NKp80/KLRF1 C-Terminus
  Val66-Tyr231
  Accession # Q9NZS2
• Specificity: Detects human NKp80/KLRF1 C-Terminus in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
  1% cross‑reactivity with recombinant human (rh) NKp44 and rhNKp30 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KLRF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp80/KLRF1 Antibody [Unconjugated] - C-terminus

• Activating coreceptor NKp80
• CLEC5C
• CLEC5CMGC119908
• C-type lectin domain family 5 member C
• killer cell lectin-like receptor F1
• killer cell lectin-like receptor subfamily F member 1
• killer cell lectin-like receptor subfamily F, member 1
• KLRF1
• Lectin-like receptor F1
• MGC119907
• MGC119909
• ML
• NKp80

Background

NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating co‑receptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1‑3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD) (SwissProt # Q9NZS2). The ECD contains the C-type lectin-like domain (aa 114‑230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). One early study suggested that NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor
tyrosine‑based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region, and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2‑3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2, 3). One study reports that NKp80 likely functions as a co‑receptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80‑AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).

1. Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
2. Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
4. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
5. Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKp80/KLRF1 Antibody (AF1900)(1)

Publication using AF1900

• B Bauer, T Wotapek, T Zöller, E Rutkowski, A Steinle The Activating C-type Lectin-like Receptor NKp65 Signals through a Hemi-immunoreceptor Tyrosine-based Activation Motif (hemITAM) and Spleen Tyrosine Kinase (Syk) J. Biol. Chem, 2017-01-12;292(8):3213-3223. 2017-01-12 [PMID: 28082678] (Western Blot, Human)

Bioinformatics

• Gene Symbol: KLRF1
• Uniprot:
  • Q9NZS2(Human)