Cleavage stimulation factor 2 Antibody

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human and mouse Cleavage stimulation factor 2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSTF64 antibody NB100-57530 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human CSTF64 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CSTF64 (NB100-57530) used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human CSTF64 (Cleavage stimulation factor 2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSTF64 antibody NB100-57530 used for IP at 3 ug per reaction. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57531. For blotting immunoprecipitated CSTF64, NB100-57530 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cleavage stimulation factor 2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 375 and 425 of human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa using the numbering given in entry NP_001316.1 (GeneID 1478).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSTF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody is useful in Western Blot and immunoprecipitation.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cleavage stimulation factor 2 Antibody

• betaCstF-64 variant 2
• CF-1 64 kDa subunit
• Cleavage stimulation factor 64 kDa subunit
• cleavage stimulation factor subunit 2
• cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD
• cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• CSTF 64 kDa subunit
• CstF-64

Background

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSTF2
• Entrez:
  • 1478(Human)
• Uniprot:
  • NP_001316.1(Human)