Claudin-3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody [NBP2-48526] - Staining of human cell line MCF7 shows localization to cell junctions. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Claudin-3 Antibody [NBP2-48526] - Staining of human duodenum shows strong membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Claudin-3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Claudin-3 Antibody - BSA Free (NBP2-48526) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: PREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Claudin-3 Recombinant Protein Antigen (NBP2-48526PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Claudin-3 Antibody - BSA Free

• C7orf1
• claudin 3
• Claudin3
• Claudin-3
• CLDN3
• Clostridium perfringens enterotoxin receptor 2
• CPE-R 2
• CPE-R2
• CPETR2
• CPETR2CPE-receptor 2
• HRVP1
• Rat ventral prostate.1 protein homolog
• RVP1
• ventral prostate.1 protein homolog
• ventral prostate.1-like protein

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN3