CKAP1 Antibody - Azide and BSA Free

Immunoprecipitation: CKAP1 Antibody [NBP3-00264] - TBCB was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg HuT78 Whole Cell Lysate 2 uL NBP3-00264 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00264, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa

Western Blot: CKAP1 Antibody [NBP3-00264] - NBP3-00264 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: NCI-H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:35 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CKAP1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human CKAP1 (Uniprot#: Q99426; Met1-Ile244)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBCB
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 0.5-2 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CKAP1 Antibody - Azide and BSA Free

• CG22Tubulin-specific chaperone B
• CKAP1MGC14625
• CKAPI
• cytoskeleton associated protein 1
• Cytoskeleton-associated protein 1Cytoskeleton-associated protein CKAPI
• tubulin folding cofactor B
• tubulin-folding cofactor B

Background

CKAP1 binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathwayleading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimerdissociation. May function as

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBCB