14-3-3 eta Antibody (3G12)

Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - HEK293T, MDA-MB-231 and MCF-7 whole cell lysates, 30 ug/lane. 10% SDS-PAGE. Antibody at 1:1000, 4C overnight. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: 14-3-3 eta Antibody (3G12) [NBP1-92691] - Analysis of HeLa cells stained with mouse mAb to 14.3.3, NBP1-92691, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The NBP1-92691 antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region.

Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - analysis of neural tissue and cell lysates using Mouse mAb against 14-3-3n ( green). [1] protein standard, [2] Rat whole brain, [3] Mouse whole brain, [4] NIH/3T3, [5] Hek293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Eq
• Applications: WB, ICC/IF, IHC, KO
• Clone: 3G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

14-3-3 eta Antibody (3G12) Summary

• Immunogen: Full length recombinant human 14-3-3 eta expressed in and purified from E.coli [UniProt# Q04917].
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Knockout Validated
  • Western Blot 1:5000
• Application Notes: This 14-3-3 eta (3G12) antibody is useful for Immunocytochemistry/Immunofluorescence, immunohistochemistry, and Western blot, where a band can be seen at approximately 28 kDa. Knockout validation (PMID: 31640562).
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 14-3-3 eta Antibody (3G12)

• 1433 eta
• 14-3-3 eta
• 14-3-3 protein eta
• Protein AS1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
• YWHA1
• YWHA114-3-3 eta
• YWHAH

Background

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities. 14-3-3 eta has been shown to accumulate in CSF of Creutzfeld Jacob Disease patients; bind to alpha-synuclein in Lewy bodies of Parkinson's disease, and has been linked to early-onset Schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 eta Antibody (NBP1-92691)(3)

Publications using NBP1-92691

• Eisa AA, De S, Detwiler A et al. YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation BMC Dev. Biol. 2019-10-22 [PMID: 31640562] (KO, WB, Mouse)
• Eisa AA ROLE OF 14-3-3 ETA AND EPSILON IN GAMETOGENESIS Thesis (WB, Mouse)
• Trimova G, Yamagata K, Iwata S et al. Tumour necrosis factor alpha promotes secretion of 14-3-3n by inducing necroptosis in macrophages Arthritis Res. Ther. 2020-02-12 [PMID: 32051018] (ICC/IF, Human)

Review for 14-3-3 eta Antibody (NBP1-92691) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/20/2021

Summary

• Application: Western Blot
• Sample Tested: 293T and MCF-7 cells-whole cell lysate,MDA-231 cell lysate
• Species: Human
• Lot: 061217

Bioinformatics

• Gene Symbol: YWHAH
• Entrez:
  • 7533(Human)
• Uniprot:
  • Q04917()