CKAP1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CKAP1 Antibody [NBP1-85671] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Immunohistochemistry-Paraffin: CKAP1 Antibody [NBP1-85671] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Western Blot: CKAP1 Antibody [NBP1-85671] - Analysis using Anti-TBCB antibody NBP1-85671 (A) shows similar pattern to independent antibody NBP1-85672 (B).

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CKAP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CKAP1 Antibody - BSA Free (NBP1-85671) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VSAPTVTVFISSSLNTFRSEKRYSRSLTIAEFKCKLELLVGSPASCMELELYGVDDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGARLGEYEDVSR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBCB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CKAP1 Protein (NBP1-85671PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CKAP1 Antibody - BSA Free

• CG22Tubulin-specific chaperone B
• CKAP1MGC14625
• CKAPI
• cytoskeleton associated protein 1
• Cytoskeleton-associated protein 1Cytoskeleton-associated protein CKAPI
• tubulin folding cofactor B
• tubulin-folding cofactor B

Background

CKAP1 binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathwayleading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimerdissociation. May function as

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBCB