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CKAP1 Antibody

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Western Blot: CKAP1 Antibody [NBP1-33462] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: CKAP1 Antibody [NBP1-33462] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CKAP1 Antibody [NBP1-33462] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CKAP1 Antibody [NBP1-33462] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.
Western Blot: CKAP1 Antibody [NBP1-33462] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CKAP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CKAP1. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBCB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for CKAP1 Antibody

  • CG22Tubulin-specific chaperone B
  • CKAP1MGC14625
  • CKAPI
  • cytoskeleton associated protein 1
  • Cytoskeleton-associated protein 1Cytoskeleton-associated protein CKAPI
  • tubulin folding cofactor B
  • tubulin-folding cofactor B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Gigaxonin Antibody [NBP2-33569] - Immunofluorescent staining of human cell line RT4 shows localization to microtubules.
Immunohistochemistry-Paraffin: Gigaxonin Antibody [NBP2-33569] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
TBCE Antibody
NBP1-81711
Western Blot: TBCE Antibody [NBP1-81711] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TBCE Antibody [NBP1-81711] - Staining of human placenta shows strong positivity in trophoblastic cells.
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Applications: WB, IHC, IHC-P
CHAF1A Antibody
(SS 1 1-13)
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Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).
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Dynactin Subunit
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Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
IKBKAP Antibody
NBP1-76788
Western Blot: IKBKAP Antibody [NBP1-76788] - A-20 cell lysate with IKBKAP antibody at in (A) 0.5, and (B) 1 u/mL.
Immunocytochemistry: IKBKAP Antibody [NBP1-76788] - A-20 cells with IKBKAP antibody at 1 ug/ml.
Immunofluorescence: IKBKAP Antibody [NBP1-76788] - Immunofluorescence of IKAP in A20 cells with IKAP antibody at 20 ug/mL.
Species: Hu, Mu
Applications: WB, ELISA, ICC, IF
HMBS Antibody
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Western Blot: HMBS Antibody [NBP2-33600] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: HMBS Antibody [NBP2-33600] - Staining of human cell line A-431 shows localization to lipid droplets.
Immunohistochemistry-Paraffin: HMBS Antibody [NBP2-33600] - Staining in human bone marrow and cerebral cortex tissues using anti-HMBS antibody. Corresponding HMBS RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
c-Abl Antibody
[Unconjugated]
AF5414
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Applications: WB, Simple Western
Aminopeptidase
N/CD13 Antibody
NBP2-33855
Western Blot: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Analysis in human small intestine tissue.
Immunocytochemistry/Immunofluorescence: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Staining of human cell line BJ shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody [NBP2-33855] - Staining in human small intestine and cerebral cortex tissues using anti-ANPEP antibody. Corresponding ANPEP RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
CTR9 Antibody
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Western Blot: CTR9 Antibody [NB100-68205] - Detection of Human and Mouse CTR9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTR9 antibody NB100-68205 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: CTR9 Antibody [NB100-68205] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Western Blot: CTR9 Antibody [NB100-68205] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-68205 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Species: Hu, Mu
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Vimentin Antibody
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Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - analysis of HeLa cell culture stained with chicken pAb to vimentin, NB300-223, dilution 1:10,000 in green, and costained with EnCor mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates using chicken pAb to Vimentin, NB300-223, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
(1 Review)
(22 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF, ICC
alpha Tubulin
Antibody (DM1A)
NB100-690
Western Blot: alpha Tubulin Antibody (DM1A) [NB100-690] - Analysis of HeLa and COS-7 lysates.
Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [NB100-690] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-alpha Tubulin [DM1A] conjugated to Alexa Fluor 488 [NB100-690AF488] at 2ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [NB100-690] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse prostate using alpha Tubulin antibody (clone DM1A) at 1:200 dilution. The signal was developed using HRP labelled secondary and DAB reagent which followed counterstaining with hematoxylin. The antibody generated a specific cytoplasmic/cytoskeletal staining in the prostate epithelial cells.
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready, Flow-IC, IF
PIK3R4 Antibody
NBP1-30463
Western Blot: PIK3R4 Antibody [NBP1-30463] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Vps15 was also immunoprecipitated by rabbit anti-Vps15 antibody NBP1-30462.
(4 Publications)
Species: Hu, Mu, Ze
Applications: WB, ICC/IF, IP
14-3-3 eta
Antibody (3G12)
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Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - analysis of neural tissue and cell lysates using Mouse mAb against 14-3-3n ( green). [1] protein standard, [2] Rat whole brain, [3] Mouse whole brain, [4] NIH/3T3, [5] Hek293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells.
Immunocytochemistry/Immunofluorescence: 14-3-3 eta Antibody (3G12) [NBP1-92691] - Analysis of HeLa cells stained with mouse mAb to 14.3.3, NBP1-92691, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The NBP1-92691 antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
MAP1B [p Thr1265]
Antibody - SuperBUGS
NBP1-42827
Western Blot: MAP1B [p Thr1265] Antibody (SuperBUGS) [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).
Immunohistochemistry: MAP1B [p Thr1265] Antibody (SuperBUGS) [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.
(8 Publications)
Species: Hu, Mu, Rt, Ch, Fi, Re, Xp
Applications: WB, ICC/IF, IHC, IHC-Fr
Neuronal Pentraxin
1 Antibody
NBP2-62710
Immunohistochemistry-Paraffin: Neuronal Pentraxin 1 Antibody [NBP2-62710] - Staining of human cerebral cortex shows strong synapse staining and cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Neuronal Pentraxin 1 Antibody [NBP2-62710] - Analysis in human cerebral cortex and tonsil tissues using Anti-NPTX1 antibody. Corresponding NPTX1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Neuronal Pentraxin 1 Antibody [NBP2-62710] - Staining of human cerebral cortex shows high expression.
Species: Hu
Applications: IHC, IHC-P
Doublecortin
Antibody (3E1)
NBP1-92684
Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
Glutathione S
Transferase kappa 1 A ...
NBP1-89727
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining in human small intestine and pancreas tissues using anti-GSTK1 antibody. Corresponding GSTK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
CKAP1 Antibody
NBP1-33462
CKAP1 Antibody
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol TBCB