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CKAP1 Antibody

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Western Blot: CKAP1 Antibody [NBP1-33462] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: CKAP1 Antibody [NBP1-33462] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CKAP1 Antibody [NBP1-33462] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CKAP1 Antibody [NBP1-33462] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.
Western Blot: CKAP1 Antibody [NBP1-33462] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CKAP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CKAP1. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBCB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for CKAP1 Antibody

  • CG22Tubulin-specific chaperone B
  • CKAP1MGC14625
  • CKAPI
  • cytoskeleton associated protein 1
  • Cytoskeleton-associated protein 1Cytoskeleton-associated protein CKAPI
  • tubulin folding cofactor B
  • tubulin-folding cofactor B

Background

CKAP1 binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathwayleading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimerdissociation. May function as

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-49924
Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. GIG pulls down overexpressed PRPH in a Kelch-dependent manner. Cell lysates were subjected to immunoprecipitation with anti-Flag beads or IgG, and the membrane was probed sequentially for PRPH then GIG (not shown). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY licence.
Western Blot: Gigaxonin Antibody [NBP1-49924] - The Kelch domain of GIG interacts with intermediate filament proteins. Endogenous VIM pulls down endogenous GIG. Indicated lysates were subjected to immunoprecipitation with either VIM antibody or control IgG (used as the mock condition throughout), and the membrane was probed sequentially for endogenous GIG and then VIM (not shown). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140157), licensed under a CC-BY licence.
Immunoprecipitation: Gigaxonin Antibody [NBP1-49924] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Gigaxonin antibody NBP1-49924 used for IP at 3 ug/mg lysate. Gigaxonin was also immunoprecipitated by rabbit anti-Gigaxonin antibody NBP1-49923, which recognizes an upstream epitope. For blotting immunoprecipitated Gigaxonin, NBP1-49924 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
(1 Publication)
Species: Hu
Applications: IP, WB
TBCE Antibody
H00006905-B01P
Western Blot: TBCE Antibody [H00006905-B01P] - Analysis of TBCE expression in HepG2.
Western Blot: TBCE Antibody [H00006905-B01P] - Analysis of TBCE expression in transfected 293T cell line by TBCE polyclonal antibody. Lane 1: TBCE transfected lysate(57.97 KDa). Lane 2: Non-transfected lysate.
Western Blot: TBCE Antibody [H00006905-B01P] - Analysis of TBCE expression in human spleen.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, WB
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(SS 1 1-13)
NB500-207
Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).
(10 Publications)
Species: Hu
Applications: ICC/IF, IP, WB
Dynactin Subunit
1/DCTN1 Antibody
NB100-1110
Flow Cytometry: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
(3 Publications)
Species: Hu, Mu, Rb
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, PLA, WB
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(6G9)
H00008518-M03
ELISA: IKBKAP Antibody (6G9) [H00008518-M03] - Detection limit for recombinant GST tagged IKBKAP is approximately 0.3ng/ml as a capture antibody.
Immunohistochemistry-Paraffin: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of monoclonal antibody to IKBKAP on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
Western Blot: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of IKBKAP expression in HeLa (Cat # L013V1).
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HMBS Antibody
NBP2-33600
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Immunohistochemistry-Paraffin: HMBS Antibody [NBP2-33600] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: HMBS Antibody [NBP2-33600] - Staining in human bone marrow and cerebral cortex tissues using anti-HMBS antibody. Corresponding HMBS RNA-seq data are presented for the same tissues.
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
c-Abl Antibody
[Unconjugated]
AF5414
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c‑Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(1 Publication)
Species: Hu
Applications: Simple Western, WB
Aminopeptidase
N/CD13 Antibody [Unco ...
AF2335
Aminopeptidase N/CD13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(26 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, IP, WB
CTR9 Antibody
NB100-68205
Immunoprecipitation: CTR9 Antibody [NB100-68205] - Detection of human CTR9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTR9 antibody NB100-68205 (lot NB100-68205-4) used for IP at 6 ug per reaction. CTR9 was also immunoprecipitated by a previous lot of this antibody (lot NB100-68205-3). For blotting immunoprecipitated CTR9, NB100-68205 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CTR9 used at a dilution of 1:250.
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
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NB300-223
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. IHC-Fr image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. ICC/IF image submitted by a verified customer review.
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(56 Publications)
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
alpha Tubulin
Antibody (DM1A) - BSA ...
NB100-690
Flow (Intracellular): alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody (DM1A) NB100-690AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image from the Alexa Fluor 488 version of this antibody.
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
Flow Cytometry: alpha Tubulin Antibody (DM1A) [NB100-690] - An intracellular stain was performed on HeLa cells with alpha Tubulin [DM1A] Antibody NB100-690AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(14 Reviews)
(107 Publications)
Species: Av, Bv, Ca, Ch, ChHa, Dr, Fu, Gt, Gp, Ha, Hu, Pm, Mu, Pa, Po, Pm, Rb, Rt, Xp, Ye
Applications: CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, Simple Western, WB
PIK3R4 Antibody
NBP1-30463
Western Blot: PIK3R4 Antibody [NBP1-30463] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Vps15 was also immunoprecipitated by rabbit anti-Vps15 antibody NBP1-30462.
(12 Publications)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IP, WB
14-3-3 eta
Antibody (3G12)
NBP1-92691
Immunocytochemistry/Immunofluorescence: 14-3-3 eta Antibody (3G12) [NBP1-92691] - Analysis of HeLa cells stained with mouse mAb to 14.3.3, NBP1-92691, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The NBP1-92691 antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region.
Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - HEK293T, MDA-MB-231 and MCF-7 whole cell lysates, 30 ug/lane. 10% SDS-PAGE. Antibody at 1:1000, 4C overnight. WB image submitted by a verified customer review.
Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - analysis of neural tissue and cell lysates using Mouse mAb against 14-3-3n ( green). [1] protein standard, [2] Rat whole brain, [3] Mouse whole brain, [4] NIH/3T3, [5] Hek293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells.
(1 Review)
(3 Publications)
Species: Bv, Ca, Eq, Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
MAP1B [p Thr1265]
Antibody - SuperBUGS
NBP1-42827
Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Western blot analysis of p62, Nbr1, MAP1B-HC, and MAP1B-LC1 protein levels following blockage of autophagic protein degradation. Phospho-MAP1B-HC is degraded by autophagy. Upon blockage of autophagic degradation with Bafilomycin A1: (Baf), levels of phospho-MAP1B-HC increase compared with levels of total MAP1B-HC. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/ijcb/2012/208014/), licensed under a CC-BY licence.
Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).
Immunohistochemistry: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.
(8 Publications)
Species: Ch, Fi, Hu, Pm, Mu, Rt, Re, Xp
Applications: ICC/IF, IHC, IHC-Fr, WB
Neuronal Pentraxin
1
7707-NP
Neuronal Pentraxin 1
(1 Publication)
Species: Hu
Applications: BA
Doublecortin
Antibody
NBP1-72042
Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL).
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Glutathione S
Transferase kappa 1 A ...
NBP2-16686
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Wild-type (WT) and Glutathione S Transferase kappa 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione S Transferase kappa 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CKAP1 Antibody
NBP1-33462
CKAP1 Antibody
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol TBCB