Cited-2 Antibody [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cited-2 at approximately 30-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cited-2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cited-2 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cited-2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cited-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Cited-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Cited-2
  Met1-Cys269
  Accession # O35740
• Specificity: Detects mouse Cited-2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CITED2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-10 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cited-2 Antibody [Unconjugated]

• Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain2,cbp/p300-interacting transactivator 2
• Cited2
• Cited-2
• MRG-1
• MRG1melanocyte-specific gene 1-related gene 1
• MSG1-related gene 1
• MSG-related protein 1
• P35srj

Background

Cited-2 is a 270 amino acid protein that functions by binding to the CH1 region of CREB binding protein (CBP)/p300. This binding interferes with certain transcription factors like HIF1 alpha and STAT2 blocking their ability to bind and interact with CBP/p300. Cited-2 knockout mice die due to defects in heart and neural tube development. Mutations in Cited-2 have been reported in patients with congenital heart defects. It appears Cited-2 modulates apoptosis by a p53 independent pathway whereby the details still need to be elucidated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cited-2 Antibody (AF5005)(5)

Publications using AF5005

• Jorge López-Tello, Vicente Pérez-García, Jaspreet Khaira, Laura C Kusinski, Wendy N Cooper, Adam Andreani et al. Fetal and trophoblast PI3K p110 alpha have distinct roles in regulating resource supply to the growing fetus in mice eLife 6/26/2019 [PMID: 31241463]
• Swaathi Jayaraman, Michele Doucet, Scott L. Kominsky CITED2 attenuates macrophage recruitment concordant with the downregulation of CCL20 in breast cancer cells Oncology Letters 11/15/2017 [PMID: 29399152]
• Li Q, Hakimi P, Liu X, Yu W, Ye F, Fujioka H, Raza S, Shankar E, Tang F, Dunwoodie S, Danielpour D, Hoppel C, Ramirez-Bergeron D, Qu C, Hanson R, Yang Y Cited2, a transcriptional modulator protein, regulates metabolism in murine embryonic stem cells. J Biol Chem, 2013-11-21;289(1):251-63. 2013-11-21 [PMID: 24265312] (ChIP, Mouse)
• Li Q, Ramirez-Bergeron D, Dunwoodie S, Yang Y Cited2 gene controls pluripotency and cardiomyocyte differentiation of murine embryonic stem cells through Oct4 gene. J Biol Chem, 2012-07-03;287(34):29088-100. 2012-07-03 [PMID: 22761414] (ChIP, Western Blot, Human)
• Du J, Chen Y, Li Q, Han X, Cheng C, Wang Z, Danielpour D, Dunwoodie SL, Bunting KD, Yang YC HIF-1 alpha deletion partially rescues defects of hematopoietic stem cell quiescence caused by Cited2 deficiency. Blood, 2012-02-02;119(12):2789-98. 2012-02-02 [PMID: 22308296] (ChIP, Mouse)

Review for Cited-2 Antibody (AF5005) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ChIP Mouse 01/08/2015

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: See PMID 22308296
• Species: Mouse

Bioinformatics

• Gene Symbol: CITED2
• Uniprot:
  • O35740()