CILP-1 Antibody [Unconjugated]

Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CILP-1 N-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CILP-1 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.2 mg/mL. A specific band was detected for CILP‑1 at approximately 133 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CILP‑1 N-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5504) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the66-440 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CILP-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CILP‑1 N-Terminal Fragment
  Arg22-Arg720
  Accession # NP_003604
• Specificity: Detects human CILP‑1 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CILP-1 C-terminal peptide is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CILP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CILP-1 Antibody [Unconjugated]

• cartilage intermediate layer protein 1 C1
• cartilage intermediate layer protein 1 C2
• cartilage intermediate layer protein 1
• cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
• Cartilage intermediate-layer protein
• CILP
• CILP1
• CILP-1
• HsT18872

Background

The CILP-1 (cartilage intermediate-layer protein 1) gene product is a 132 kDa (predicted) monomeric glycoprotein that is found in both hyaline and fibrocartilage. It is a precursor for two secreted, proteolytically generated products, a 90 kDa N-terminal CILP-1, and a 62 kDa C-terminal NTPPHase-homolog. The N-terminus is suggested to serve as both a matrix structural protein, and an IGF-I/TGF-beta 1 suppressor sequestration molecule. Human CILP-1 spans aa 22‑720 of the CILP-1 precursor. It contains one TSP-1 domain (aa 149‑201), a C2‑type Ig-like region (aa 309‑395) and six potential N-glycosylation sites. Over aa 1‑720 of the CILP-1 precursor, human CILP-1 shares 89% aa identity with mouse CILP‑1, and 42% aa identity with human CILP-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CILP-1 Antibody (AF5504)(1)

Publication using AF5504

• FA van Nieuwe, C Munts, RC Op't Veld, A González, J Díez, S Heymans, B Schroen, M van Bilsen Cartilage intermediate layer protein 1 (CILP1): A novel mediator of cardiac extracellular matrix remodelling Sci Rep, 2017-11-22;7(1):16042. 2017-11-22 [PMID: 29167509] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CILP
• Uniprot:
  • NP_003604()